Department of Anatomy, National University of Ireland, Galway, Ireland.
Cytotherapy. 2010 May;12(3):313-25. doi: 10.3109/14653241003695018.
Spinal cord injury is a devastating injury that impacts drastically on the victim's quality of life. Stem cells have been proposed as a therapeutic strategy. Neural stem (NS) cells have been harvested from embryonic mouse forebrain and cultured as adherent cells. These NS cells express markers of neurogenic radial glia.
Mouse NS cells expressing green fluorescent protein (GFP) were transplanted into immunosupressed rat spinal cords following moderate contusion injury at T9. Animals were left for 2 and 6 weeks then spinal cords were fixed, cryosectioned and analyzed. Stereologic methods were used to estimate the volume and cellular environment of the lesions. Engraftment, migration and differentiation of NS cells were also examined.
NS cells integrated well into host tissue and appeared to migrate toward the lesion site. They expressed markers of neurons, astrocytes and oligodendrocytes at 2 weeks post-transplantation and markers of neurons and astrocytes at the 6-week time-point. NS cells appeared to have a similar morphologic phenotype to radial glia, in particular at the pial surface.
Although no functional recovery was observed using the Basso Beattie Bresnahan (BBB) locomotor rating scale, NS cells are a potential cellular therapy for treatment of injured spinal cord. They may be used as delivery vehicles for therapeutic proteins because they show an ability to migrate toward the site of a lesion. They may also be used to replace lost or damaged neurons and oligodendrocytes.
脊髓损伤是一种破坏性损伤，极大地影响了受害者的生活质量。干细胞已被提议作为一种治疗策略。神经干细胞（NS）已从胚胎鼠前脑中分离出来，并作为贴壁细胞进行培养。这些 NS 细胞表达神经发生放射状胶质的标志物。
在 T9 处中度挫伤损伤后，将表达绿色荧光蛋白（GFP）的小鼠 NS 细胞移植到免疫抑制大鼠脊髓中。动物被放置 2 周和 6 周后，固定、冷冻切片并进行分析。立体学方法用于估计病变的体积和细胞环境。还检查了 NS 细胞的植入、迁移和分化。
NS 细胞很好地整合到宿主组织中，并似乎向病变部位迁移。它们在移植后 2 周表达神经元、星形胶质细胞和少突胶质细胞的标志物，在 6 周时表达神经元和星形胶质细胞的标志物。NS 细胞的形态表型似乎与放射状胶质细胞相似，特别是在脑表面。
尽管使用 Basso Beattie Bresnahan（BBB）运动评分量表没有观察到功能恢复，但 NS 细胞是治疗受伤脊髓的潜在细胞治疗方法。它们可以用作治疗性蛋白质的递送载体，因为它们显示出向病变部位迁移的能力。它们也可用于替代丢失或受损的神经元和少突胶质细胞。
