Miyazaki T, Dharmarajan A M, Atlas S J, Katz E, Wallach E E
Department of Gynecology and Obstetrics, John Hopkins University School of Medicine, Baltimore, Maryland.
Fertil Steril. 1991 Jun;55(6):1183-8. doi: 10.1016/s0015-0282(16)54372-6.
To determine if prostaglandins (PGs) have a direct effect on the ovarian proteolytic enzyme system by examining ultrastructure of the follicle wall and the microvasculature in the presence and absence of indomethacin and by using the isolated perfused rabbit ovary.
Nine hours after administration of human chorionic gonadotropin (hCG) or hCG plus indomethacin, follicles were removed and processed for scanning and transmission electron microscopy. Isolated perfused rabbit ovaries were induced to ovulate with PGF2 alpha (100 ng/mL) in the presence and absence of tranexamic acid (0.1, 1.0, or 10 mM), a plasminogen activator inhibitor.
The addition of indomethacin to hCG inhibited ovulation and production of PGs without affecting the follicular microvasculature. However, the changes in follicle wall architecture were less pronounced after treatment with indomethacin. Ovulatory efficiency in response to PGF2 alpha (the percent of follicles greater than 1.5 mm that ovulate) was significantly reduced (P less than 0.01) by 10 mM tranexamic acid.
These results suggest that PGs induce follicular rupture by activation of proteolytic enzymes located in the follicle wall.
通过在有和没有吲哚美辛的情况下检查卵泡壁和微血管的超微结构，并使用离体灌注兔卵巢，来确定前列腺素（PGs）是否对卵巢蛋白水解酶系统有直接影响。
在给予人绒毛膜促性腺激素（hCG）或hCG加吲哚美辛9小时后，取出卵泡并进行扫描和透射电子显微镜检查。在有和没有氨甲环酸（0.1、1.0或10 mM）（一种纤溶酶原激活物抑制剂）的情况下，用前列腺素F2α（100 ng/mL）诱导离体灌注兔卵巢排卵。
在hCG中添加吲哚美辛可抑制排卵和PGs的产生，而不影响卵泡微血管。然而，用吲哚美辛处理后，卵泡壁结构的变化不太明显。10 mM氨甲环酸可使对前列腺素F2α的排卵效率（大于1.5 mm的卵泡排卵的百分比）显著降低（P<0.01）。
这些结果表明，PGs通过激活位于卵泡壁的蛋白水解酶来诱导卵泡破裂。
