Marine Natural Products Laboratory, Korea Ocean Research and Development Institute, Ansan, Seoul 425-600, Korea.
J Microbiol Biotechnol. 2010 Mar;20(3):501-5.
1-Acetyl-beta-carboline was isolated as an anti-MRSA agent from the fermentation broth of a marine actinomycete isolated from marine sediment. The producing strain was identified to be Streptomyces sp. by phylogenetic analysis of the 16S rRNA gene sequence. The anti-MRSA agent was isolated by bioactivity-guided fractionation of the culture extract by solvent partitioning, ODS open flash chromatography, and purification with a reversed-phase HPLC. Its structure was elucidated by extensive 2D NMR and mass spectral analyses. Combination of 1-acetyl-beta- carboline with ampicillin exhibited synergistic antibacterial activity against MRSA.
1-乙酰-β-咔啉是从海洋沉积物中分离出的海洋放线菌发酵液中分离得到的抗耐甲氧西林金黄色葡萄球菌（MRSA）药物。通过对 16S rRNA 基因序列的系统发育分析，确定产生菌为链霉菌属。采用溶剂分配、ODS 开管快速色谱和反相 HPLC 等方法对培养物提取物进行生物活性导向分离，分离出抗 MRSA 药物。通过广泛的二维 NMR 和质谱分析确定了其结构。1-乙酰-β-咔啉与氨苄西林联合使用表现出协同抗菌活性，对 MRSA 具有抗菌活性。
