Plohl M, Gamulin V
Department of Organic Chemistry and Biochemistry, Ruder Bosković Institute, University of Zagreb, Croatia, Yugoslavia.
FEMS Microbiol Lett. 1991 Jan 15;61(2-3):139-43. doi: 10.1016/0378-1097(91)90541-h.
One Streptomyces rimosus ribosomal RNA gene set (rrnF) was cloned into pBR322 as 8.8 kilobase-pairs BamHI fragment and sequence of the 3' end of the operon with 5S rRNA gene was determined. 5S rRNA gene is 120 bp long and highly homologous with two other known 5S rRNA from Streptomyces. Remarkable sequence homology with S. ambofaciens rrnD operon exists in the 3' noncoding regions, including first transcription termination signal. S. rimosus rrnF operon contains second putative terminator which is absent in S. ambofaciens rrnD operon. tRNA genes were not found at the 3' end of rrnF operon. The number of rRNA operons in S. rimosus was defined by Southern hybridization analysis. S. rimosus possess six rRNA operons and all rRNA operons contain identical PstI fragment of 4.5 kb. rRNA genes in operons are separated by short intergenic regions and organized in the order 16S-23S-5S. Efforts to connect tRNA genes with S. rimosus rRNA operons were unsuccessful.
将一个天蓝色链霉菌核糖体RNA基因集（rrnF）作为8.8千碱基对的BamHI片段克隆到pBR322中，并测定了带有5S rRNA基因的操纵子3'末端的序列。5S rRNA基因长度为120 bp，与来自链霉菌的另外两个已知5S rRNA高度同源。在3'非编码区，包括第一个转录终止信号，与产二素链霉菌rrnD操纵子存在显著的序列同源性。天蓝色链霉菌rrnF操纵子包含第二个假定的终止子，而产二素链霉菌rrnD操纵子中不存在该终止子。在rrnF操纵子的3'末端未发现tRNA基因。通过Southern杂交分析确定了天蓝色链霉菌中rRNA操纵子的数量。天蓝色链霉菌拥有六个rRNA操纵子，所有rRNA操纵子都包含4.5 kb的相同PstI片段。操纵子中的rRNA基因由短的基因间隔区隔开，并按16S-23S-5S的顺序排列。将tRNA基因与天蓝色链霉菌rRNA操纵子连接的尝试未成功。
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