诺卡氏链霉菌ATCC 14899中rRNA操纵子的分子克隆及比较序列分析

Molecular cloning and comparative sequence analyses of rRNA operons in Streptomyces nodosus ATCC 14899.

作者信息

Yap W H, Wang Y

机构信息

Microbial Collection and Screening Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, 30 Medical Drive, Singapore 11760, Singapore.

出版信息

Gene. 1999 May 17;232(1):77-85. doi: 10.1016/s0378-1119(99)00112-2.

DOI:10.1016/s0378-1119(99)00112-2

PMID:10333524

Abstract

The genome of Streptomyces nodosus contains six ribosomal RNA (rRNA) operons. Four of the rRNA operons; rrnB, rrnD, rrnE and rrnF were cloned. We have completely sequenced all four operons, including a region 750 base pairs (bp) upstream of the 16S rRNA gene. The three rRNA genes present in each operon were closely linked in the order 16S-23S-5S. A sequence comparison of the four operons showed more than 99% sequence similarity between the corresponding 16S and 23S rRNA genes, and more than 97% similarity between 5S rRNA genes. The sequence differences observed between 23S rRNA genes appeared to be localized in two specific regions. Substantial sequence differences were found in the region upstream of the 16S rRNA gene as well as in the internal transcribed spacers. No tRNA gene was found in the 16S-23S spacer regions.

摘要

诺卡氏链霉菌的基因组包含六个核糖体RNA（rRNA）操纵子。其中四个rRNA操纵子，即rrnB、rrnD、rrnE和rrnF被克隆出来。我们已对所有四个操纵子进行了完整测序，包括16S rRNA基因上游750个碱基对（bp）的区域。每个操纵子中存在的三个rRNA基因按16S - 23S - 5S的顺序紧密相连。对这四个操纵子的序列比较显示，相应的16S和23S rRNA基因之间的序列相似性超过99%，5S rRNA基因之间的相似性超过97%。在23S rRNA基因之间观察到的序列差异似乎集中在两个特定区域。在16S rRNA基因上游区域以及内部转录间隔区也发现了显著的序列差异。在16S - 23S间隔区未发现tRNA基因。