单核细胞增生李斯特菌核糖体RNA操纵子的研究。

Studies on the ribosomal RNA operons of Listeria monocytogenes.

作者信息

Thompson D E, Balsdon J T, Cai J, Collins M D

机构信息

Department of Microbiology, AFRC Institute of Food Research, Reading, UK.

出版信息

FEMS Microbiol Lett. 1992 Sep 15;75(2-3):219-24. doi: 10.1016/0378-1097(92)90407-f.

DOI:10.1016/0378-1097(92)90407-f

PMID:1383082

Abstract

A 23S rRNA gene of Listeria monocytogenes was cloned into pUC19 on a 6.2-kb Pst I fragment. Hybridisation studies demonstrated the presence of the 5S and partial 16S rRNA genes within the clone. The nucleotide sequence of the region encoding the 23S rRNA was found to be highly homologous with those of other low G + C Gram-positive bacteria. The 16S-23S intergenic spacer region was amplified using PCR technology and revealed two product sizes, the larger of which contained tRNA(Ala) and tRNA(Ileu) genes. Further tRNA genes were found downstream of the 5S rRNA gene.

摘要

将单核细胞增生李斯特菌的一个23S rRNA基因克隆到pUC19载体上的一个6.2kb Pst I片段中。杂交研究表明该克隆中存在5S和部分16S rRNA基因。发现编码23S rRNA区域的核苷酸序列与其他低G+C革兰氏阳性菌的序列高度同源。利用PCR技术扩增16S-23S基因间隔区，发现有两种产物大小，其中较大的产物包含tRNA(Ala)和tRNA(Ileu)基因。在5S rRNA基因下游还发现了其他tRNA基因。