Johnson J L, Bastian N R, Schauer N L, Ferry J G, Rajagopalan K V
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
FEMS Microbiol Lett. 1991 Jan 15;61(2-3):213-6. doi: 10.1016/0378-1097(91)90554-n.
The pterin cofactor in formate dehydrogenase isolated from Methanobacterium formicium is identified as molybdopterin guanine dinucleotide. The pterin, stabilized as the alkylated, dicarboxamidomethyl derivative, is shown to have absorption and chromatographic properties identical to those of the previously characterized authentic compound. Treatment with nucleotide pyrophosphatase produced the expected degradation products GMP and carboxyamidomethyl molybdopterin. The molybdopterin guanine dinucleotide released from the enzyme by treatment with 95% dimethyl sulfoxide is shown to be functional in the in vitro reconstitution of the cofactor-deficient nitrate reductase in extracts of the Neurospora crassa nit-1 mutant.
从甲酸甲烷杆菌中分离出的甲酸脱氢酶中的蝶呤辅因子被鉴定为钼蝶呤鸟嘌呤二核苷酸。该蝶呤以烷基化的二羧酰胺甲基衍生物形式稳定存在，其吸收和色谱性质与先前表征的纯品化合物相同。用核苷酸焦磷酸酶处理产生了预期的降解产物GMP和羧酰胺甲基钼蝶呤。用95%二甲基亚砜处理从酶中释放出的钼蝶呤鸟嘌呤二核苷酸，在体外重建粗糙脉孢菌nit-1突变体提取物中缺乏辅因子的硝酸还原酶时显示出功能性。
