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Molybdoenzyme biosynthesis in Escherichia coli: in vitro activation of purified nitrate reductase from a chlB mutant.大肠杆菌中钼酶的生物合成：来自chlB突变体的纯化硝酸还原酶的体外激活
J Bacteriol. 1992 Dec;174(24):7934-40. doi: 10.1128/jb.174.24.7934-7940.1992.
2
Involvement of a low-molecular-weight substance in in vitro activation of the molybdoenzyme respiratory nitrate reductase from a chlB mutant of Escherichia coli.一种低分子量物质参与来自大肠杆菌chlB突变体的钼酶呼吸硝酸盐还原酶的体外激活。
J Bacteriol. 1987 Oct;169(10):4678-85. doi: 10.1128/jb.169.10.4678-4685.1987.
3
Activation in vitro of respiratory nitrate reductase of Escherichia coli K12 grown in the presence of tungstate. Involvement of molybdenum cofactor.在钨酸盐存在下生长的大肠杆菌K12呼吸硝酸盐还原酶的体外激活。钼辅因子的参与。
Eur J Biochem. 1986 Aug 1;158(3):483-90. doi: 10.1111/j.1432-1033.1986.tb09780.x.
4
Identification of the molybdenum cofactor in chlorate-resistant mutants of Escherichia coli.大肠杆菌抗氯酸盐突变体中钼辅因子的鉴定
J Bacteriol. 1981 Oct;148(1):274-82. doi: 10.1128/jb.148.1.274-282.1981.
5
Involvement of a protein with molybdenum cofactor in the in vitro activation of nitrate reductase from a chlA mutant of Escherichia coli K12.一种含钼辅因子的蛋白质参与大肠杆菌K12 chlA突变体硝酸还原酶的体外激活。
Biochim Biophys Acta. 1987 Aug 21;914(3):220-32. doi: 10.1016/0167-4838(87)90281-0.
6
Isolation of protein FA, a product of the mob locus required for molybdenum cofactor biosynthesis in Escherichia coli.蛋白质FA的分离，FA是大肠杆菌中钼辅因子生物合成所需的mob基因座的产物。
Eur J Biochem. 1994 Jun 1;222(2):687-92. doi: 10.1111/j.1432-1033.1994.tb18913.x.
7
Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.大肠杆菌K-12中的硝酸还原酶：chlC、chlE和chlG基因座的作用。
J Bacteriol. 1982 Aug;151(2):788-99. doi: 10.1128/jb.151.2.788-799.1982.
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Molybdenum cofactor in chlorate-resistant and nitrate reductase-deficient insertion mutants of Escherichia coli.大肠杆菌耐氯酸盐和硝酸盐还原酶缺陷插入突变体中的钼辅因子。
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Molybdenum cofactor requirement for in vitro activation of apo-molybdoenzymes of Escherichia coli.大肠杆菌脱辅基钼酶体外激活对钼辅因子的需求
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Molybdenum cofactor: a compound in the in vitro activation of both nitrate reductase and trimethylamine-N-oxide reductase activities in Escherichia coli K12.钼辅因子：一种参与大肠杆菌K12中硝酸还原酶和三甲胺-N-氧化物还原酶活性体外激活的化合物。
Biochim Biophys Acta. 1986 Aug 15;872(3):243-52. doi: 10.1016/0167-4838(86)90277-3.

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The Role of the Nucleotides in the Insertion of the bis-Molybdopterin Guanine Dinucleotide Cofactor into apo-Molybdoenzymes.核苷酸在双钼喋呤鸟嘌呤二核苷酸辅酶插入脱辅基钼酶中的作用。
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Evolution of light-independent protochlorophyllide oxidoreductase.不依赖光的原叶绿素酸酯氧化还原酶的进化
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The hydrogenases and formate dehydrogenases of Escherichia coli.大肠杆菌的氢化酶和甲酸脱氢酶。
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Association of molybdopterin guanine dinucleotide with Escherichia coli dimethyl sulfoxide reductase: effect of tungstate and a mob mutation.钼蝶呤鸟嘌呤二核苷酸与大肠杆菌二甲基亚砜还原酶的关联：钨酸盐和mob突变的影响
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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.大肠杆菌K-12中的硝酸还原酶：chlC、chlE和chlG基因座的作用。
J Bacteriol. 1982 Aug;151(2):788-99. doi: 10.1128/jb.151.2.788-799.1982.
3
Nitrate reductase and cytochrome bnitrate reductase structural genes as parts of the nitrate reductase operon.硝酸还原酶和细胞色素b硝酸还原酶结构基因作为硝酸还原酶操纵子的组成部分。
Mol Gen Genet. 1981;181(4):535-40. doi: 10.1007/BF00428749.
4
Characterization of the molybdenum cofactor of sulfite oxidase, xanthine, oxidase, and nitrate reductase. Identification of a pteridine as a structural component.亚硫酸盐氧化酶、黄嘌呤氧化酶和硝酸还原酶的钼辅因子的特性。蝶啶作为结构成分的鉴定。
J Biol Chem. 1980 Mar 10;255(5):1783-6.
5
The pterin component of the molybdenum cofactor. Structural characterization of two fluorescent derivatives.钼辅因子的蝶呤成分。两种荧光衍生物的结构表征。
J Biol Chem. 1984 May 10;259(9):5414-22.
6
A common pathway for the activation of several molybdoenzymes in Escherichia coli K12.大肠杆菌K12中几种钼酶激活的共同途径。
Biochim Biophys Acta. 1984 Apr 10;798(2):216-25. doi: 10.1016/0304-4165(84)90307-6.
7
Identification and purification of a protein involved in the activation of nitrate reductase in the soluble fraction of a chlA mutant of Escherichia coli K12.
Biochim Biophys Acta. 1983 Nov 28;749(1):115-24. doi: 10.1016/0167-4838(83)90158-9.
8
Phenotypic restoration by molybdate of nitrate reductase activity in chlD mutants of Escherichia coli.钼酸盐对大肠杆菌chlD突变体硝酸还原酶活性的表型恢复作用
J Bacteriol. 1971 Nov;108(2):854-60. doi: 10.1128/jb.108.2.854-860.1971.
9
[Study of chlorate resistant mutants of Escherichia coli K 12. I. Reconstitution in vitro of particulate nitrate reductase activity of Escherichia coli K 12].[大肠杆菌K12耐氯酸盐突变体的研究。I. 大肠杆菌K12颗粒状硝酸还原酶活性的体外重建]
Biochim Biophys Acta. 1969 Feb 11;171(2):238-52. doi: 10.1016/0005-2744(69)90157-0.
10
Cloning and nucleotide sequence of the chlD locus.chlD基因座的克隆与核苷酸序列
J Bacteriol. 1987 May;169(5):1911-6. doi: 10.1128/jb.169.5.1911-1916.1987.

大肠杆菌中钼酶的生物合成：来自chlB突变体的纯化硝酸还原酶的体外激活

Molybdoenzyme biosynthesis in Escherichia coli: in vitro activation of purified nitrate reductase from a chlB mutant.

作者信息

Santini C L, Iobbi-Nivol C, Romane C, Boxer D H, Giordano G

机构信息

Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseille, France.

出版信息

J Bacteriol. 1992 Dec;174(24):7934-40. doi: 10.1128/jb.174.24.7934-7940.1992.

DOI:10.1128/jb.174.24.7934-7940.1992
PMID:1459941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207528/
Abstract

All molybdoenzyme activities are absent in chlB mutants because of their inability to synthesize molybdopterin guanine dinucleotide, which together with molybdate constitutes the molybdenum cofactor in Escherichia coli. The chlB mutants are able to synthesize molybdopterin. We have previously shown that the inactive nitrate reductase present in a chlB mutant can be activated in a process requiring protein FA and a heat-stable low-molecular-weight substance. We show here that purified nitrate reductase from the soluble fraction of a chlB mutant can be partially activated in a process that requires protein FA, GTP, and an additional protein termed factor X. It appears that the molybdopterin present in the nitrate reductase of a chlB mutant is converted to molybdopterin guanine dinucleotide during activation. The activation is absolutely dependent upon both protein FA and factor X. Factor X activity is present in chlA, chlB, chlE, and chlG mutants.

摘要

由于chlB突变体无法合成钼蝶呤鸟嘌呤二核苷酸，所有钼酶活性在chlB突变体中均不存在，钼蝶呤鸟嘌呤二核苷酸与钼酸盐共同构成大肠杆菌中的钼辅因子。chlB突变体能够合成钼蝶呤。我们之前已经表明，chlB突变体中存在的无活性硝酸还原酶可以在一个需要蛋白质FA和一种热稳定的低分子量物质的过程中被激活。我们在此表明，从chlB突变体的可溶部分纯化的硝酸还原酶可以在一个需要蛋白质FA、GTP和另一种称为因子X的蛋白质的过程中被部分激活。似乎chlB突变体的硝酸还原酶中存在的钼蝶呤在激活过程中会转化为钼蝶呤鸟嘌呤二核苷酸。这种激活绝对依赖于蛋白质FA和因子X。因子X活性存在于chlA、chlB、chlE和chlG突变体中。