Al'nikin A F, Dzhusupova A B, Akhverdian V Z, Krylov V N
Genetika. 1991 Jan;27(1):39-50.
More than 170 phage-resistant mutants (PRM) of the first order of Pseudomonas putida strain PpG1 were obtained using newly isolated and previously described bacteriophages specific for this strain. According to the results of analysis of resistance of the mutants to each of 31 phages of PpG1 strain and 8 phages of the PpN strain, the PRM strains were distributed into 20 groups. In most cases, the reason for resistance is loss of absorption capacity of bacteria. However, no direct relation between the level of absorption and efficiency of phage plating was detected. It was shown that some of the PRM of P. putida PpG1 strains acquired the ability to maintain the growth of phages specific for the other P. putida strain, PpN. Frequencies of isolating mutants of various resistance types depend on the concrete phage used. In accordance with their absorption specificity, all phages were distributed into 23 groups, and a tridimensional formal scheme of receptor sites for these phages on the PpG1 strain was drawn. In the process of selection of the PpG1 clones resistant to non-lysogenizing mutant of temperate PP71 phage, a variant of this strain manifesting the phenomenon of "auto-plaquing" was found. These results support the mutational origin of this phenomenon in some cases.
利用新分离的以及先前描述的对恶臭假单胞菌菌株PpG1具有特异性的噬菌体，获得了170多个该菌株的一级噬菌体抗性突变体（PRM）。根据对这些突变体对PpG1菌株的31种噬菌体和PpN菌株的8种噬菌体的抗性分析结果，PRM菌株被分为20组。在大多数情况下，抗性的原因是细菌吸收能力的丧失。然而，未检测到吸收水平与噬菌体平板接种效率之间的直接关系。结果表明，恶臭假单胞菌PpG1菌株的一些PRM获得了维持对另一恶臭假单胞菌菌株PpN具有特异性的噬菌体生长的能力。不同抗性类型突变体的分离频率取决于所使用的具体噬菌体。根据它们的吸收特异性，所有噬菌体被分为23组，并绘制了这些噬菌体在PpG1菌株上受体位点的三维形式图。在筛选对温和噬菌体PP71的非溶原化突变体具有抗性的PpG1克隆的过程中，发现了该菌株的一个表现出“自噬斑形成”现象的变体。这些结果在某些情况下支持了这种现象的突变起源。
