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恶臭假单胞菌噬菌体的形态学和基因组结构研究及其分类

[Study of morphology and genome structure of Pseudomonas putida bacteriophages for their classification].

作者信息

Krylov V N, Dzhusupova A B, Akhverdian V Z, Khrenova E A, Al'nikin A F, Kopylova Iu I

出版信息

Genetika. 1989 Sep;25(9):1559-70.

PMID:2599372
Abstract

A group of 27 bacteriophages specific for Pseudomonas putida strains PpG1 and PpN has been isolated. The phages were characterized and compared with the previously described virulent (pf 16, af, tf and PMW) and temperate (PP56 and PP71) phages. The new phages belong to B1 and C1 morphotypes, according to Ackerman's classification. Phage DNAs were digested with several endonucleases; the molecular weights and homology of the DNAs were determined. All phages of P. putida isolated up to now were distributed into 10 species (groups), on the basis of particle morphology, genome size and the results of homology studies. Recombination processes are believed to participate in formation of phages belonging to certain species.

摘要

已分离出一组针对恶臭假单胞菌菌株PpG1和PpN的27种噬菌体。对这些噬菌体进行了表征,并与先前描述的烈性噬菌体(pf 16、af、tf和PMW)和温和噬菌体(PP56和PP71)进行了比较。根据阿克曼分类法,新噬菌体属于B1和C1形态型。用几种核酸内切酶消化噬菌体DNA;测定了DNA的分子量和同源性。根据颗粒形态、基因组大小和同源性研究结果,目前分离出的所有恶臭假单胞菌噬菌体被分为10个种(组)。据信重组过程参与了某些种噬菌体的形成。

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