Krylov V N, Rasskazchikova S A, Al'nikin A F
Genetika. 1996 Mar;32(3):348-53.
The efficiency of using bacteria in open systems to degrade different anthropogenic toxic pollutants can depend strongly on the interaction between these bacteria and natural bacteriophages. The possibility of selecting bacterial Pseudomonas putida mutants resistant to all bacteriophages of this species known so far was tested (in our work, these mutants were designated totally phage-resistant mutants). In a model experiment, changes in the composition of a population upon prolonged growth of bacteria in the presence of one of the virulent phages were examined. On the basis of the results obtained, it is postulated that: (1) Mutants differing in resistance to various phages accumulate in a population; relative numbers of different mutants can undergo alterations over the course of time; mutants selected in the presence of a given virulent phage do not often manifest complete resistance to this phage. (2) It is possible to isolate totally phage-resistant mutants of P. putida PpG1. These mutants carry up to three different mutations simultaneously; however, these mutants regain sensitivity to many phages upon pseudoreversion occurrence.
在开放系统中利用细菌降解不同人为有毒污染物的效率，可能在很大程度上取决于这些细菌与天然噬菌体之间的相互作用。我们测试了筛选对目前已知的该物种所有噬菌体均具有抗性的恶臭假单胞菌突变体的可能性（在我们的研究中，这些突变体被称为完全抗噬菌体突变体）。在一个模型实验中，研究了在一种烈性噬菌体存在的情况下细菌长时间生长时种群组成的变化。根据所得结果推测：（1）对不同噬菌体具有不同抗性的突变体在种群中积累；不同突变体的相对数量可能会随时间发生变化；在给定烈性噬菌体存在下筛选出的突变体通常不会对该噬菌体表现出完全抗性。（2）有可能分离出恶臭假单胞菌PpG1的完全抗噬菌体突变体。这些突变体同时携带多达三种不同的突变；然而，这些突变体在发生假回复时会重新对许多噬菌体恢复敏感。
