Chemical Engineering Department, Izmir Institute of Technology, Izmir 35430, Turkey.
J Mater Sci Mater Med. 2010 Jul;21(7):2227-36. doi: 10.1007/s10856-010-4065-x. Epub 2010 Apr 7.
Chitosan films incorporated with thyme oil for potential applications of wound dressing were successfully prepared by solvent casting method. The water vapor permeability, oxygen transmission rate, and mechanical properties of the films were determined. Surface and cross-section morphologies and the film thicknesses were determined by Scanning Electron Microscopy (SEM). Fourier transform infrared (FT-IR) spectroscopy was conducted to determine functional group interactions between the chitosan and thyme oil. Thermal behaviors of the films were analyzed by Thermal Gravimetry (TGA) and Differential Scanning Calorimetry (DSC). In addition, the antimicrobial and the antioxidant activities of the films were investigated. The antimicrobial test was carried by agar diffusion method and the growth inhibition effects of the films including different amount of thyme oil were tested on the gram negative microorganisms of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and a gram positive microorganism of Staphylococcus aureus. The minimum thyme oil concentration in chitosan films showing the antimicrobial activity on all microorganisms used in the study was found as 1.2 % (v/v). In addition, this concentration showed the highest antioxidant activity due to mainly the carvacrol in thyme oil. Water vapor permeability and oxygen transmission rate of the films slightly increased, however, mechanical properties decreased with thyme oil incorporation. The results revealed that the thyme oil has a good potential to be incorporated into chitosan to make antibacterial and permeable films for wound healing applications.
壳聚糖薄膜中添加了百里香精油，成功地通过溶剂浇铸法制备，可潜在应用于伤口敷料。对薄膜的水蒸气透过率、氧气透过率和力学性能进行了测定。通过扫描电子显微镜（SEM）测定了薄膜的表面和横截面形态以及薄膜厚度。傅里叶变换红外（FT-IR）光谱用于确定壳聚糖和百里香精油之间的功能基团相互作用。通过热重（TGA）和差示扫描量热法（DSC）分析了薄膜的热行为。此外，还研究了薄膜的抗菌和抗氧化活性。通过琼脂扩散法进行了抗菌试验，研究了不同含量的百里香精油对革兰氏阴性菌大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌和革兰氏阳性菌金黄色葡萄球菌的生长抑制作用。结果发现，在研究中使用的所有微生物上具有抗菌活性的壳聚糖薄膜中百里香精油的最小浓度为 1.2%（v/v）。此外，由于百里香精油中的主要成分是香芹酚，该浓度还表现出了最高的抗氧化活性。水蒸气透过率和氧气透过率略有增加，但是，随着百里香精油的添加，力学性能下降。结果表明，百里香精油具有很好的潜力，可以被掺入壳聚糖中，用于制作具有抗菌和透气性能的伤口愈合应用的薄膜。
