Department of Internal Medicine and Rehabilitation Science, Tohoku University Graduate School of Medicine, Aoba-Ku, Sendai, Japan.
Clin Exp Hypertens. 2010 Jan;32(2):76-83. doi: 10.3109/10641960902993046.
Physical fitness is closely related to cardiovascular health. We examined the effects of estradiol, angiotensin-converting enzyme inhibitor, exercise training, and their combination on exercise capacity as well as skeletal muscle fiber type and capillarity in old female rats. Twelve-month-old female Wistar-Kyoto rats were allocated to six groups: control (C), treatment with 17 beta-estradiol (0.025 mg/kg/dose, i.p. twice a week) (Est), perindopril (2 mg/kg/day) (Per), exercise training on a treadmill (15 m/min, 10 grade incline, 60 min/day, 5 days/week) (Exe), and combinations of a drug and exercise training (Exe+Est and Exe+Per). Following 6-month interventions, the rats were submitted to a stepwise exercise test on a treadmill. Moreover, fiber type and capillarity in both the soleus and gastrocnemius muscles were examined. Exercise capacity, capillary density, and the percentage of type I fiber significantly increased in Exe, Exe+Est, and Exe+Per compared to C. There were no significant differences in exercise capacity, capillary density, and percentage of type I fiber among C, Est, and Per. The combination of exercise training and perindopril further increased capillary density in both the soleus and gastrocnemius muscles, and the percentage of type I fiber in the gastrocnemius muscle compared to exercise training alone. We found that in old female rats, chronic treatment with estradiol or perindopril affected neither untrained exercise capacity nor exercise capacity acquired as a result of exercise training. However, we found that perindopril promotes adaptive changes of skeletal muscle in response to exercise such as increases in capillary density and the percentage of type I fiber.
身体适应能力与心血管健康密切相关。我们检测了雌二醇、血管紧张素转换酶抑制剂、运动训练及其联合应用对老年雌性大鼠运动能力、骨骼肌纤维类型和毛细血管的影响。12 月龄雌性 Wistar-Kyoto 大鼠被分为 6 组：对照组（C）、17β-雌二醇（0.025mg/kg/剂量，腹腔内注射，每周两次）治疗组（Est）、培哚普利（2mg/kg/天）治疗组（Per）、跑步机运动训练组（15m/min，10 级坡度，每天 60min，每周 5 天）（Exe）以及药物与运动训练联合组（Exe+Est 和 Exe+Per）。经过 6 个月的干预，大鼠在跑步机上进行逐步运动试验。此外，还检测了比目鱼肌和腓肠肌的纤维类型和毛细血管密度。与 C 组相比，Exe、Exe+Est 和 Exe+Per 组的运动能力、毛细血管密度和 I 型纤维百分比显著增加。C、Est 和 Per 组之间的运动能力、毛细血管密度和 I 型纤维百分比无显著差异。与单独运动训练相比，运动训练与培哚普利联合应用进一步增加了比目鱼肌和腓肠肌的毛细血管密度和腓肠肌的 I 型纤维百分比。我们发现，在老年雌性大鼠中，慢性雌二醇或培哚普利治疗既不影响未训练的运动能力，也不影响运动训练获得的运动能力。然而，我们发现培哚普利促进了骨骼肌对运动的适应性变化，如毛细血管密度和 I 型纤维百分比的增加。
