Boll I
Klin Wochenschr. 1978 Feb 15;56(4):187-95. doi: 10.1007/BF01705382.
The Effects of Various Substances in the Culture Microenvironment on the Proliferation of Erythroblasts in vitro were Studied and Evaluated by differential cell and mitosis counting in normal and pathologic bone marrow. The mitotic frequency and maturation correlated directly with the erythropoietin content of the medium and were potentiated on addition of either folic acid, etiocholanolon or cAMP. However, erythroblast proliferation was stimulated independently of erythropoietin concentration when either cobalt, and androgen or an anabolic substance was added to the medium. An iron-deficient medium prevented the maturation of erythroblasts to reticulocytes, thereby rendering erythropoiesis ineffective. The existence of an erythrocyte chalone ineffective. The existence of an erythrocyte chalone or an erythropoietin inhibitor could not be deduced from our experiments since the transformation of pluripotent to erythropoetin-sensitive stem cells is not included. A stimulation of the granulopoietic proliferation pool occurred when serum from patients with either polycythemia vera or after acute blood loss was added to the medium.
通过对正常和病理骨髓中的细胞差异计数和有丝分裂计数，研究并评估了培养微环境中各种物质对体外成红细胞增殖的影响。有丝分裂频率和成熟度与培养基中的促红细胞生成素含量直接相关，并且在添加叶酸、本胆烷醇酮或环磷酸腺苷（cAMP）后会增强。然而，当向培养基中添加钴、雄激素或合成代谢物质时，成红细胞增殖的刺激与促红细胞生成素浓度无关。缺铁培养基会阻止成红细胞成熟为网织红细胞，从而使红细胞生成无效。红细胞抑素的存在无效。由于未包括多能干细胞向促红细胞生成素敏感干细胞的转化，因此无法从我们的实验中推断出红细胞抑素或促红细胞生成素抑制剂的存在。当将真性红细胞增多症患者或急性失血后患者的血清添加到培养基中时，粒细胞生成增殖池会受到刺激。
