多重耐药鲍曼不动杆菌 AbaR 型基因组耐药岛的进化。
Evolution of AbaR-type genomic resistance islands in multiply antibiotic-resistant Acinetobacter baumannii.
机构信息
School of Molecular and Microbial Biosciences, The University of Sydney, NSW 2006, Australia.
出版信息
J Antimicrob Chemother. 2010 Jun;65(6):1162-70. doi: 10.1093/jac/dkq095. Epub 2010 Apr 7.
OBJECTIVES
To determine if members of the European clonal lineages are present amongst multiply antibiotic-resistant Acinetobacter baumannii isolates from Australia. To search for AbaR-type genomic antibiotic resistance islands and determine the genetic organization of any AbaR detected.
METHODS
Two groups of multiply antibiotic-resistant A. baumannii strains isolated between 2002 and 2007 at three Sydney hospitals were studied. Various molecular techniques were used to determine whether they belong to European epidemic clones and to detect and characterize the AbaR.
RESULTS
One group of five isolates belonged to European clone I. In these, an AbaR-type island, characterized by the presence of resistance genes and Tn6018, was present. Mapping revealed two novel AbaR configurations, AbaR6 and AbaR7, with IS26-generated deletions of 29 and 36.2 kb, respectively, relative to AbaR5. The second group contained seven isolates belonging to European clone II and, in these, a different transposon was present in the same chromosomal location as the AbaR genomic resistance islands.
CONCLUSIONS
Multiply antibiotic-resistant A. baumannii strains belonging to both European epidemic clonal lineages I and II are found in Australian hospitals, indicating that these clones are globally distributed. Amongst the isolates examined, only the clone I strains harboured an AbaR-type resistance island, and two novel types, AbaR6 and AbaR7, carrying only four antibiotic resistance genes were identified. Hence, AbaR are continuously evolving and IS26 plays a key role in this process.
目的
确定来自澳大利亚的多重抗生素耐药鲍曼不动杆菌分离株中是否存在欧洲克隆谱系成员。搜索 AbaR 型基因组抗生素耐药岛，并确定检测到的任何 AbaR 的遗传组织。
方法
研究了 2002 年至 2007 年间在悉尼三家医院分离的两组多重抗生素耐药鲍曼不动杆菌菌株。使用各种分子技术来确定它们是否属于欧洲流行克隆，并检测和表征 AbaR。
结果
一组五个分离株属于欧洲克隆 I。在这些分离株中，存在 AbaR 型岛，其特征是存在耐药基因和 Tn6018。图谱显示，与 AbaR5 相比，存在两种新型 AbaR 构型 AbaR6 和 AbaR7，分别具有 29 和 36.2 kb 的 IS26 生成缺失。第二组包含属于欧洲克隆 II 的七个分离株，在这些分离株中，相同染色体位置存在不同的转座子与 AbaR 基因组耐药岛相同。
结论
属于欧洲流行克隆谱系 I 和 II 的多重抗生素耐药鲍曼不动杆菌菌株在澳大利亚医院中发现，表明这些克隆在全球范围内分布。在所检查的分离株中，只有克隆 I 菌株携带 AbaR 型耐药岛，并且鉴定出两种新型 AbaR6 和 AbaR7，仅携带四个抗生素耐药基因。因此，AbaR 不断进化，IS26 在这一过程中发挥关键作用。
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