Genomic Analysis of Isolates Carrying OXA-23 and OXA-58 Genes from Animals Reveals ST1 and ST25 as Major Clonal Lineages.

is increasingly being recognized as a relevant pathogen for animals with a putative zoonotic impact. This study aimed at identifying and characterizing carbapenemase-producing from animals. Among 503 , mainly isolated from dogs/cats (75.7%) between 2013 and 2018, 42 isolates from 22 veterinary clinics (VCs) harboured ( = 29) or ( = 13). The gene was located on plasmids (11.4-21.1 kb) within different genetic surroundings (patterns A-D). was embedded in Tn on the chromosome ( = 4; pattern a) or Tn on plasmids ( = 9; 41.2-71.3 kb; patterns b-e). The predominant IC1-ST1-OXA-58 (66.7%; 96.4% cgMLST complex type (CT)-1808) was disseminated among 11 VCs in Germany. Resistance islands R3-like ( = 15) and R10 ( = 1) have emerged among ST1-isolates since 2016. IC7-ST25-OXA-23 isolates (21.4%) occurred in seven VCs in Germany, France and Italy and differed in their resistance gene patterns from those of OXA-58 isolates. They were separated into six CTs, basically according to their regional origin. Other STs observed were ST10, ST578 and ST602. In conclusion, OXA-23 and OXA-58 were linked with ST1 and ST25, two globally distributed lineages in humans. The suggested transmission of certain lineages within and among VCs together with the acquisition of R islands hints at a successful dissemination of multidrug-resistant strains in the VC environment.