口疮病毒属马鼻炎 A 病毒的细胞进入依赖于内体酸化。
Cell entry of the aphthovirus equine rhinitis A virus is dependent on endosome acidification.
机构信息
Institute of Molecular and Cellular Biology and Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom.
出版信息
J Virol. 2010 Jun;84(12):6235-40. doi: 10.1128/JVI.02375-09. Epub 2010 Apr 7.
Abstract
Equine rhinitis A virus (ERAV) is genetically closely related to foot-and-mouth disease virus (FMDV), and both are now classified within the genus Aphthovirus of the family Picornaviridae. For disease security reasons, FMDV can be handled only in high-containment facilities, but these constraints do not apply to ERAV, making it an attractive alternative for the study of aphthovirus biology. Here, we show, using immunofluorescence, pharmacological agents, and dominant negative inhibitors, that ERAV entry occurs (as for FMDV) via clathrin-mediated endocytosis and acidification of early endosomes. This validates the use of ERAV as a model system to study the mechanism of cell entry by FMDV.
摘要
马鼻炎病毒（ERAV）在基因上与口蹄疫病毒（FMDV）密切相关，两者现在都被归类为小 RNA 病毒科口疮病毒属。出于疾病安全的原因，只能在高生物安全设施中处理 FMDV，但这些限制不适用于 ERAV，这使其成为研究口疮病毒生物学的有吸引力的替代选择。在这里，我们通过免疫荧光、药理学试剂和显性负抑制剂显示，ERAV 的进入（如 FMDV 一样）是通过网格蛋白介导的内吞作用和早期内体酸化发生的。这验证了使用 ERAV 作为模型系统来研究 FMDV 细胞进入机制的用途。
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