牛痘病毒角膜炎中免疫细胞浸润的动力学。

Kinetics of immune cell infiltration in vaccinia virus keratitis.

机构信息

Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706, USA.

出版信息

Invest Ophthalmol Vis Sci. 2010 Sep;51(9):4541-8. doi: 10.1167/iovs.09-5107. Epub 2010 Apr 7.

DOI:10.1167/iovs.09-5107

PMID:20375330

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2941182/

Abstract

PURPOSE

Vaccinia virus keratitis leading to blindness is a severe complication of smallpox vaccination. The clinical manifestations of vaccinia virus keratitis are similar to those of herpes simplex virus keratitis, a well-studied immunopathologic disease. Vaccinia virus keratitis is likely to involve an immunopathologic component, but little is known about the pathogenesis of the disease. The goal of this study was to determine type and kinetics of immune cell infiltration in the cornea during vaccinia virus keratitis.

METHODS

Rabbit eyes were trephined and inoculated with 1x10(5) pfu of the Dryvax strain of the vaccinia virus. On days 2, 4, 7, 10, 14, and 28 after infection, the animals were scored for clinical disease and eye sections were stained for B cells, CD4+ cells, CD8+ cells, and neutrophils. The eyelid, ciliary body, cornea, iris, iridocorneal angle, and choroid were examined.

RESULTS

Corneal vaccinia virus challenge resulted in the infiltration of B cells, CD4+ cells, CD8+ cells, and neutrophils into the cornea and eyelids. Neutrophils were the predominant cell type on days 2 and 3 after infection, whereas CD4+ cells were the predominant cell type detected in corneas on days 4 through 10. CD8+ cells and B cells peaked on day 10, but at lower levels than CD4+ cells and neutrophils.

CONCLUSIONS

These results suggest that sequential migration of neutrophils, then CD4+ cells, plays an important role in vaccinia virus keratitis.

摘要

目的

导致失明的牛痘病毒角膜炎是天花疫苗接种的严重并发症。牛痘病毒角膜炎的临床表现与疱疹单纯病毒角膜炎相似，后者是一种研究充分的免疫病理疾病。牛痘病毒角膜炎可能涉及免疫病理成分，但对该病的发病机制知之甚少。本研究的目的是确定在牛痘病毒角膜炎中角膜内免疫细胞浸润的类型和动力学。

方法

用 1x10(5) pfu 的 Dryvax 株牛痘病毒对兔眼进行环钻和接种。在感染后第 2、4、7、10、14 和 28 天，对动物进行临床疾病评分，并对眼部分别进行 B 细胞、CD4+细胞、CD8+细胞和中性粒细胞染色。检查眼睑、睫状体、角膜、虹膜、虹膜角膜角和脉络膜。

结果

角膜牛痘病毒攻击导致 B 细胞、CD4+细胞、CD8+细胞和中性粒细胞浸润到角膜和眼睑。中性粒细胞是感染后第 2 和第 3 天的主要细胞类型，而 CD4+细胞是感染后第 4 至 10 天在角膜中检测到的主要细胞类型。CD8+细胞和 B 细胞在第 10 天达到高峰，但水平低于 CD4+细胞和中性粒细胞。