定量兔带状疱疹角膜炎模型。
A quantitative rabbit model of vaccinia keratitis.
机构信息
Department of Medical Microbiology and ImmunologyUniversity of Wisconsin-Madison, Madison, WI 53706, USA.
出版信息
Invest Ophthalmol Vis Sci. 2010 Sep;51(9):4531-40. doi: 10.1167/iovs.09-5106. Epub 2010 Apr 7.
PURPOSE
The goal of this study was to use multiple quantitative disease measures to evaluate the effect of various viral inocula on the development of vaccinia keratitis in rabbits.
METHODS
Trephined eyes of female rabbits were infected with 10(4), 10(5), 10(6), or 10(7) plaque-forming units (pfu) of the Dryvax strain of the vaccinia virus and scored daily for disease for 14 days according to a modification of the MacDonald-Shadduck scoring system. Ocular viral titers and vaccinia-specific antibody titers were determined by plaque assay and ELISA, respectively.
RESULTS
The amount of virus used for infection affected the severity of disease, with 10(4) pfu eliciting milder keratitis after delayed onset compared with higher amounts of virus. At inocula above 10(5) pfu the course and severity of corneal disease was not significantly different. The time to reach peak titers was delayed in the 10(4) group but peak titers were similar in all groups. Severe conjunctival chemosis interfered with scoring in animals infected with 10(6) or 10(7) pfu. Virus-specific antibody titers were similar in all groups at day 14. Body weights decreased less than 10% in all groups.
CONCLUSIONS
The course of vaccinia keratitis in rabbits paralleled that in humans. A viral inoculum of 10(5) pfu/eye was determined to be optimal for use in further studies of vaccinia keratitis.
目的
本研究的目的是使用多种定量疾病指标来评估不同病毒接种物对兔痘角膜炎发展的影响。
方法
用 10(4)、10(5)、10(6)或 10(7)噬菌斑形成单位(pfu)的 Dryvax 株痘苗病毒感染女性兔的环钻眼，并根据 MacDonald-Shadduck 评分系统的修改版每天对疾病进行评分 14 天。通过噬菌斑测定和 ELISA 分别测定眼内病毒滴度和痘苗特异性抗体滴度。
结果
感染用病毒量影响疾病严重程度，与使用较高量病毒相比，10(4) pfu 引起的迟发性角膜炎较轻。在接种量高于 10(5) pfu 时，角膜疾病的过程和严重程度没有显著差异。在 10(4)组中达到峰值滴度的时间延迟，但所有组的峰值滴度相似。严重的结膜水肿会干扰用 10(6)或 10(7) pfu 感染的动物的评分。所有组在第 14 天的病毒特异性抗体滴度相似。所有组的体重下降均小于 10%。
结论
兔痘角膜炎的病程与人相似。确定 10(5) pfu/眼的病毒接种量是进一步研究痘苗角膜炎的最佳选择。
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