School of Microbiology, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras.
Int J Gynecol Cancer. 2010 Apr;20(3):323-8. doi: 10.1111/IGC.0b013e3181d1c2f8.
In this study, we investigated the prevalence of human papillomavirus 16 (HPV-16) variants in Honduran women with normal cytology and with dysplasia and cervical cancer.
Samples identified as positive for HPV-16 by SPF10-LiPA were tested for intratypic subtypes and variants by analysis of the E6/E7 region using a novel reverse hybridization assay (line probe assay).
We found that most infections in all clinical groups belong to the E6 European variants, suggesting that HPV-16 non-European variants do not represent an additional factor associated with increased occurrence of high-grade cervical lesions in the studied population. Among the 106 HPV-16-positive women analyzed, E-350G was the most prevalent variant in all different disease stages, being present in 18% of cervical cancer, 13% of cervical intraepithelial neoplasia grade III (CIN III), 5% of CIN II, 5% of CIN I, and 20% of control samples. Mixed variants of HPV-16 infections were detected in 7.7% of the samples, mostly in women with normal cytology.
This study shows for the first time the diversity of HPV-16 variants in cervical samples of Honduran women.
本研究旨在调查人乳头瘤病毒 16 型（HPV-16）变异体在洪都拉斯细胞学正常、发育不良和宫颈癌女性中的流行情况。
采用 SPF10-LiPA 检测 HPV-16 阳性样本，通过新型反向杂交检测（线探针检测）分析 E6/E7 区，检测各临床组内 HPV-16 的同型内亚型和变异体。
我们发现，所有临床组中大多数感染都属于 E6 欧洲变异体，这表明 HPV-16 非欧洲变异体并非增加研究人群中高级别宫颈病变发生率的额外因素。在分析的 106 例 HPV-16 阳性妇女中，E-350G 在所有不同疾病阶段均为最常见的变异体，在宫颈癌中占 18%，CIN III 中占 13%，CIN II 中占 5%，CIN I 中占 5%，对照组样本中占 20%。在 7.7%的样本中检测到 HPV-16 混合变异体感染，主要见于细胞学正常的女性。
本研究首次显示了洪都拉斯女性宫颈样本中 HPV-16 变异体的多样性。
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