改良RPMI 1640用于贾第虫病宿主-寄生虫相互作用的体外免疫学研究。
Modification of RPMI 1640 for use in vitro immunological studies of host-parasite interactions in giardiasis.
作者信息
Guy R A, Bertrand S, Faubert G M
机构信息
Institute of Parasitology of McGill University, Macdonald College, Ste-Anne de Bellevue, Québec, Canada.
出版信息
J Clin Microbiol. 1991 Mar;29(3):627-9. doi: 10.1128/jcm.29.3.627-629.1991.
Abstract
Incubation of trophozoites for 6 h in RPMI 1640 affected the viability of the parasite; however, RPMI 1640 supplemented with L-cysteine did not affect trophozoite viability, ability to grow when transferred to fresh TYI-S-33, or ability to infect gerbils. Similarly, incubation of murine spleen cells in modified medium did not affect the viability of the cells or proliferative responses to mitogens. RPMI 1640 supplemented with 11.4 mM L-cysteine is a suitable maintenance medium for in vitro studies in immunoparasitology because it maintains viability as well as some of the physiological functions of both trophozoites and lymphocytes.
摘要
将滋养体在RPMI 1640中孵育6小时会影响寄生虫的活力；然而，添加了L-半胱氨酸的RPMI 1640不会影响滋养体的活力、转移至新鲜TYI-S-33时的生长能力或感染沙鼠的能力。同样，在改良培养基中孵育小鼠脾细胞不会影响细胞的活力或对有丝分裂原的增殖反应。添加了11.4 mM L-半胱氨酸的RPMI 1640是免疫寄生虫学体外研究的合适维持培养基，因为它能维持滋养体和淋巴细胞的活力以及一些生理功能。
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