鞭毛虫蓝氏贾第鞭毛虫的黏附作用：还原剂、血清、温度及离子组成的作用
Attachment of the flagellate Giardia lamblia: role of reducing agents, serum, temperature, and ionic composition.
作者信息
Gillin F D, Reiner D S
出版信息
Mol Cell Biol. 1982 Apr;2(4):369-77. doi: 10.1128/mcb.2.4.369-377.1982.
Abstract
The flagellated protozoan Giardia lamblia has been grown only in highly complex media under reduced oxygen tension. Therefore, the organic and physiological requirements for in vitro attachment and short-term (12-h) survival of this organism were determined. In defined maintenance media, a thiol reducing agent (e.g., cysteine) was absolutely required for attachment and survival of this aerotolerant anaerobe. The crude bovine serum Cohn III fraction greatly stimulated attachment and survival. Attachment was decreased at a reduced temperature (24 degrees C as compared with 35.5 degrees C) and absent at 12 degrees C or below. Attachment and survival were strongly dependent upon pH and ionic strength, with optima at pH 6.85 to 7.0 and 200 to 300 mosmol/kg. Sodium chloride was better tolerated than KC1. Reduction of Ca2+ and Mg2+ to below 10(-8) M did not significantly affect attachment.
摘要
鞭毛虫贾第虫仅在低氧张力下的高度复杂培养基中生长。因此，确定了该生物体体外附着和短期（12小时）存活的有机和生理需求。在限定的维持培养基中，这种耐氧厌氧菌的附着和存活绝对需要一种巯基还原剂（如半胱氨酸）。粗制牛血清科恩III级分极大地刺激了附着和存活。在较低温度下（与35.5℃相比为24℃）附着减少，在12℃或更低温度下则无附着。附着和存活强烈依赖于pH值和离子强度，最适pH值为6.85至7.0，最适离子强度为200至300 mosmol/kg。氯化钠比氯化钾更耐受。将Ca2+和Mg2+降低到10^(-8) M以下对附着没有显著影响。
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