早期播散性莱姆病患者中伯氏疏螺旋体螺旋体的直接检测。

Direct detection of Borrelia burgdorferi spirochetes in patients with early disseminated Lyme borreliosis.

作者信息

Schwarzová Katarína, Kost'anová Zina, Holecková Katarína, Spitalská Eva, Boldis Vojtech

机构信息

Institute of Virology, Slovak Academy of Sciences, Bratislava.

出版信息

Cent Eur J Public Health. 2009 Dec;17(4):179-82. doi: 10.21101/cejph.b0015.

DOI:10.21101/cejph.b0015

PMID:20377044

Abstract

The detection of spirochetes in 15 patients with clinically documented early disseminated LB has been analysed when using cultivation method of the plasma or the cerebrospinal fluid, electron microscopy, commercial Western blot and detecting the DNA of the pathogen in vitro cultures by PCR-RFLP. Spirochetes were isolated in eight blood and one cerebrospinal fluid culture samples. In seven cases (47%), previous serodiagnostic laboratory tests were negative. Borrelial DNA was detected by PCR in 67% patients (9 blood samples and 1 CSF sample). Using Msel restriction fragments of PCR products of the amplified rrf-rrl region, we identified Borrelia garinii (80%), one B. afzelii isolate and one B. burgdorferi s.s.

摘要

运用血浆或脑脊液培养法、电子显微镜检查、商用免疫印迹法以及通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测体外培养病原体的DNA，对15例临床确诊为早期播散性莱姆病（LB）的患者进行了螺旋体检测分析。在8份血液和1份脑脊液培养样本中分离出了螺旋体。7例（47%）患者之前的血清学诊断实验室检测呈阴性。67%的患者（9份血液样本和1份脑脊液样本）通过PCR检测到了伯氏疏螺旋体DNA。利用扩增的rrf-rrl区域PCR产物的Msel限制性片段，我们鉴定出了伽氏疏螺旋体（80%）、1株阿氏疏螺旋体分离株和1株狭义伯氏疏螺旋体。