Schwarzova Katarina, Kozub Peter, Szep Zoltan, Golovchenko Marina, Rudenko Natasha
Institute of Microbiology, Faculty of Medicine, Comenius University in Bratislava and University Hospital, Bratislava, Slovakia.
Department of Dermatology, Faculty Hospital with Policlinic in Nove Zamky, Nove Zamky, Slovakia.
Folia Microbiol (Praha). 2016 Sep;61(5):359-63. doi: 10.1007/s12223-016-0444-0. Epub 2016 Jan 15.
Determination of the causative agent of erythema-like skin lesions in case of nonspecific superficial perivascular dermatitis was supported by histological examination and led to the latter diagnosis of Hyperkeratosis lenticularis perstans (Flegel disease) in patient. The presence of antibodies against Borrelia burgdorferi in patient serum was confirmed by a routine ELISA method and verified by Western blot technique. Skin biopsy and blood specimens were analyzed by PCR and multilocus sequence analysis (MLSA). Western blot method revealed IgG antibody response against two specific antigens, 17 and 83 kDa proteins. The recombinant test detected IgG antibody response against p100 and p41 antigens. The sequence analysis of amplicons from the selected genomic loci obtained from skin biopsy and serum samples revealed the presence of two species from B. burgdorferi sensu lato complex as a co-infection in this patient-B. burgdorferi sensu stricto (s.s.) and Borrelia garinii.
组织学检查支持了非特异性浅表血管周围性皮炎患者红斑样皮肤病变病原体的确定，并最终诊断该患者患有持久性豆状角化过度症（弗莱格病）。通过常规酶联免疫吸附测定（ELISA）方法证实了患者血清中存在抗伯氏疏螺旋体的抗体，并通过蛋白质印迹技术进行了验证。通过聚合酶链反应（PCR）和多位点序列分析（MLSA）对皮肤活检和血液样本进行了分析。蛋白质印迹法显示针对两种特异性抗原（17 kDa和83 kDa蛋白）的IgG抗体反应。重组试验检测到针对p100和p41抗原的IgG抗体反应。对从皮肤活检和血清样本中获得的选定基因组位点的扩增子进行序列分析，结果显示该患者同时感染了伯氏疏螺旋体狭义种复合体中的两个菌种——狭义伯氏疏螺旋体（B. burgdorferi sensu stricto，s.s.）和伽氏疏螺旋体（Borrelia garinii）。
