Department of Biological and Environmental Sciences, Plant Biology, Helsinki University, Helsinki, Finland.
J Integr Plant Biol. 2010 Feb;52(2):186-94. doi: 10.1111/j.1744-7909.2010.00928.x.
Class III secretable plant peroxidases occur as a large family of genes in plants with many functions and probable redundancy. In this review we are concentrating on the evidence we have on the catalysis of lignin polymerization by class III plant peroxidases present in the apoplastic space in the xylem of trees. Some evidence exists on the specificity of peroxidase isozymes in lignin polymerization through substrate specificity studies, from antisense mutants in tobacco and poplar and from tissue and cell culture lines of Norway spruce (Picea abies) and Zinnia elegans. In addition, real time (RT-)PCR results have pointed out that many peroxidases have tissue specific expression patterns in Norway spruce. Through combining information on catalytic properties of the enzymes, on the expression patterns of the corresponding genes, and on the presence of monolignols and hydrogen peroxide in the apoplastic space, we can show that specific peroxidases catalyze lignin polymerization in the apoplastic space of Norway spruce xylem.
III 类分泌型植物过氧化物酶在植物中作为一个庞大的基因家族存在，具有多种功能和可能的冗余性。在这篇综述中，我们集中讨论了在树木木质部质外体中存在的 III 类植物过氧化物酶催化木质素聚合的证据。通过对烟草和杨树中的反义突变体以及挪威云杉（Picea abies）和百日草（Zinnia elegans）的组织和细胞培养系的底物特异性研究，有一些证据表明过氧化物酶同工酶在木质素聚合中的特异性。此外，实时（RT-）PCR 结果指出，许多过氧化物酶在挪威云杉中有组织特异性的表达模式。通过结合酶的催化特性、相应基因的表达模式以及质外体中单宁和过氧化氢的存在的信息，我们可以表明特定的过氧化物酶在挪威云杉木质部质外体中催化木质素聚合。
