Huang Chaokun, Kurotani Ken-Ichi, Tabata Ryo, Mitsuda Nobutaka, Sugita Ryohei, Tanoi Keitaro, Notaguchi Michitaka
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Bioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Hortic Res. 2023 Apr 13;10(6):uhad072. doi: 10.1093/hr/uhad072. eCollection 2023 Jun.
Grafting is a plant propagation technique widely used in agriculture. A recent discovery of the capability of interfamily grafting in has expanded the potential combinations of grafting. In this study, we showed that xylem connection is essential for the achievement of interfamily grafting and investigated the molecular basis of xylem formation at the graft junction. Transcriptome and gene network analyses revealed gene modules for tracheary element (TE) formation during grafting that include genes associated with xylem cell differentiation and immune response. The reliability of the drawn network was validated by examining the role of the () genes in TE formation during interfamily grafting. Promoter activities of and genes were found in differentiating TE cells in the stem and callus tissues at the graft junction. Analysis of a loss-of-function mutant indicated that control the timing of TE formation at the graft junction. Moreover, grafts of the overexpressor increased the scion growth rate as well as the fruit size. Thus, we identified gene modules for TE formation at the graft boundary and demonstrated potential ways to enhance interfamily grafting.
嫁接是一种在农业中广泛应用的植物繁殖技术。最近关于科间嫁接能力的发现扩展了嫁接的潜在组合。在本研究中,我们表明木质部连接对于实现科间嫁接至关重要,并研究了嫁接部位木质部形成的分子基础。转录组和基因网络分析揭示了嫁接过程中管状分子(TE)形成的基因模块,其中包括与木质部细胞分化和免疫反应相关的基因。通过研究()基因在科间嫁接过程中对TE形成的作用,验证了所绘制网络的可靠性。在嫁接部位的茎和愈伤组织中分化的TE细胞中发现了和基因的启动子活性。对一个功能缺失突变体的分析表明,控制嫁接部位TE形成的时间。此外,过表达植株的嫁接提高了接穗的生长速率以及果实大小。因此,我们确定了嫁接边界处TE形成的基因模块,并展示了增强科间嫁接的潜在方法。