Falk L, Coble B-I, Mjörnberg P-A, Fredlund H
Department of Local Health Care, County of Ostergötland, Linköping University, Linköping.
Int J STD AIDS. 2010 Apr;21(4):283-7. doi: 10.1258/ijsa.2009.009440.
The aim of the study was to evaluate the sensitivity of patients' self-sampled vaginal specimens, first-catch urine (FCU), combined vaginal/FCU specimens and endocervical specimens for detecting chlamydial infection in women. Women attending sexually transmitted disease clinics, youth clinics and a women's health clinic were enrolled. They self-collected a vaginal specimen with two swabs, which were placed into a sterile tube and into a tube containing a buffer medium, respectively. An FCU sample was collected and aliquoted into both an empty tube and the tube containing the vaginal swab. A clinician collected an endocervical swab. The samples were sent to laboratories for analysis using polymerase chain reaction testing and strand displacement amplification testing, respectively. The sensitivities calculated in all 171 Chlamydia trachomatis-infected women were equal for endocervical specimens (97.1%), vaginal specimens (96.5%) and combined vaginal/FCU specimens (95.3%), whereas the sensitivity for FCU was significantly lower (87.7%). The sensitivity of vaginal specimens for the detection of C. trachomatis is as high as that of combined vaginal/FCU specimens.
本研究的目的是评估患者自行采集的阴道标本、首次晨尿（FCU）、阴道/FCU联合标本以及宫颈管标本对检测女性衣原体感染的敏感性。纳入了在性传播疾病诊所、青少年诊所和妇女健康诊所就诊的女性。她们用两支拭子自行采集阴道标本，分别放入无菌管和含有缓冲介质的管中。采集一份FCU样本，分装到一个空管和装有阴道拭子的管中。临床医生采集宫颈管拭子。样本分别送往实验室，使用聚合酶链反应检测和链置换扩增检测进行分析。在所有171例沙眼衣原体感染女性中计算得出，宫颈管标本（97.1%）、阴道标本（96.5%）和阴道/FCU联合标本（95.3%）的敏感性相同，而FCU的敏感性显著较低（87.7%）。阴道标本检测沙眼衣原体的敏感性与阴道/FCU联合标本一样高。
