Saeed K, Pelosi E
Microbiology Department, Royal Hampshire County Hospital, Winchester, UK.
Int J STD AIDS. 2010 Apr;21(4):298-9. doi: 10.1258/ijsa.2009.009006.
Clinical diagnosis of genital herpes simplex virus (HSV) (GH) infection is insensitive and non-specific and requires laboratory confirmation. In this study we compared viral culture and the amplification of HSV DNA by polymerase chain reaction (PCR) with respect to turnaround time and cost. We compared 182 swabs submitted to our laboratory between March and May 2005, which were tested using MRC-5 cell culture, with 168 genital swabs submitted during the same months in 2006, and were tested by PCR. We concluded that PCR testing in our laboratory has significantly improved the turnaround time, with 68.4% of tests having been reported in less than 24 hours. This in turn significantly improved the service provided for the diagnosis of genital herpes without additional costs.
单纯疱疹病毒（HSV）生殖器感染的临床诊断不够敏感且缺乏特异性，需要实验室确诊。在本研究中，我们比较了病毒培养和聚合酶链反应（PCR）扩增HSV DNA在周转时间和成本方面的差异。我们将2005年3月至5月提交至我们实验室的182份拭子（采用MRC-5细胞培养进行检测）与2006年同一月份提交的168份生殖器拭子（采用PCR进行检测）进行了比较。我们得出结论，我们实验室的PCR检测显著缩短了周转时间，68.4%的检测结果在不到24小时内就已报告。这反过来又显著改善了生殖器疱疹诊断服务，且未增加额外成本。
