Foster M C, Saubermann A J
Department of Anaesthesiology, SUNY, Stony Brook 11794-8480.
J Microsc. 1991 Feb;161(Pt 2):367-73. doi: 10.1111/j.1365-2818.1991.tb03097.x.
A system based on a personal computer has been developed which provides a relatively inexpensive way to equip an electron microscopy laboratory for quantitative elemental analyses of cryosectioned biological samples. This system demonstrates the feasibility of making an X-ray analyser from a personal computer, together with commercially available hardware and software components. Hardware and software have been assembled to drive the beam in a scanning electron microscope, collect and analyse X-ray spectra, and save, retrieve, and analyse data. Our software provides a menu-controlled user interface to direct spectra acquisition and analysis. Spot analyses, video images, and quantitative elemental images may be obtained and results transferred in ASCII format to other computers. Wet weight, as well as dry weight, concentrations are calculated, if measurements were made of areas of the hydrated sample before it was freeze-dried. Grey-level copies of video and quantitative elemental images may be made on a laser printer.
已开发出一种基于个人计算机的系统，该系统为装备电子显微镜实验室以对冷冻切片生物样品进行定量元素分析提供了一种相对廉价的方法。该系统展示了利用个人计算机以及市售硬件和软件组件制造X射线分析仪的可行性。硬件和软件已组装好，用于驱动扫描电子显微镜中的电子束、收集和分析X射线光谱，以及保存、检索和分析数据。我们的软件提供了一个菜单控制的用户界面来指导光谱采集和分析。可以获得点分析、视频图像和定量元素图像，并将结果以ASCII格式传输到其他计算机。如果在冷冻干燥之前对水合样品的区域进行了测量，则可计算湿重以及干重和浓度。视频和定量元素图像的灰度副本可在激光打印机上制作。
