Equipe Environnement et Microbiologie, IPREM UMR 5254 Universite de Pau et des Pays de l'Adour, 64013 PAU, France.
Environ Sci Technol. 2010 May 1;44(9):3621-8. doi: 10.1021/es100123b.
BTEX biodegradation by an indigenous deep subsurface microbial community was evaluated in a water sample collected in the area of an underground gas storage. Five different sulfate-reducing microbial communities able to use at least either benzene, toluene, ethylbenzene, or xylene (BTEX) compounds were studied. A total of 21 different bacterial phylotypes were identified, each community containing three to nine bacterial phylotypes. Archaeal phylotypes were retrieved from only three communities. The analysis of 16S rRNA gene sequences showed that i) these consortia were mainly composed of novel species, some of which belonging to bacterial groups not previously suspected to be involved in BTEX anaerobic degradation, ii) three consortia were dominated by an uncultured Pelobacter sp. previously detected in biodegraded oil reservoirs, iii) a deeply branching species distantly affiliated to Thermotogales was abundant in two consortia, and that iv) Firmicutes related to the Desulfotomaculum and Carboxydocella genera represented the only three detectable phylotypes in a toluene-degrading consortium. This work shows that subdominant microbial populations present in a deep subsurface aquifer used for seasonal underground gas storage could be involved in the natural attenuation of the traces of BTEX coinjected with methane in the deep subsurface.
采用采集自地下储气区的水样，评估了本土深层地下微生物群落对 BTEX 的生物降解作用。研究了 5 种不同的硫酸盐还原微生物群落，它们能够利用至少苯、甲苯、乙苯或二甲苯（BTEX）中的一种化合物。共鉴定出 21 种不同的细菌类群，每个群落包含 3 至 9 种细菌类群。仅从 3 个群落中获得了古菌类群。16S rRNA 基因序列分析表明：i）这些群落主要由新物种组成，其中一些属于以前未怀疑参与 BTEX 厌氧降解的细菌群；ii）3 个群落主要由先前在生物降解油藏中检测到的未培养 Pelobacter sp. 组成；iii）与 Thermotogales 密切相关的深分支物种在两个群落中大量存在；iv）与 Desulfotomaculum 和 Carboxydocella 属相关的 Firmicutes 代表了在甲苯降解群落中唯一可检测到的 3 个类群。这项工作表明，用于季节性地下储气的深层地下含水层中存在的次要微生物种群可能参与了深部地下注入的甲烷中痕量 BTEX 的自然衰减。
