NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE).
Tohoku University.
Microbes Environ. 2020;35(4). doi: 10.1264/jsme2.ME20046.
A novel anaerobic heterotrophic strain, designated strain sy52, was isolated from a hydrothermal chimney at Suiyo Seamount in the Pacific Ocean. A 16S rRNA gene analysis revealed that the strain belonged to the family Petrotogaceae in the phylum Thermotogae. The strain was mesophilic with optimum growth at 48°C and the phylum primarily comprised hyperthermophiles and thermophiles. Strain sy52 possessed unique genomic characteristics, such as an extremely low G+C content and 6 copies of rRNA operons. Genomic analyses of strain sy52 revealed that amino acid usage in the predicted proteins resulted from adjustments to mesophilic environments. Genomic features also indicated independent adaptions to the mesophilic environment of strain sy52 and Mesotoga species, which belong to the mesophilic lineage in the phylum Thermotogae. Based on phenotypic and phylogenetic evidence, strain sy52 is considered to represent a novel genus and species in the family Petrotogaceae with the proposed name Tepiditoga spiralis gen. nov., sp. nov.
从太平洋隋尧海山的热液烟囱中分离到一株新型的厌氧异养菌株，命名为 sy52 株。16S rRNA 基因分析表明，该菌株属于热袍菌门油微菌科。该菌株是中温型的，最适生长温度为 48°C，而该门主要由嗜热菌和中温菌组成。sy52 株具有独特的基因组特征，如极低的 G+C 含量和 6 个 rRNA 操纵子。sy52 株的基因组分析表明，预测蛋白中的氨基酸使用是对中温环境的调整。基因组特征还表明，sy52 株和属于中温谱系的 Mesotoga 属的独立适应中温环境。基于表型和系统发育证据，sy52 株被认为代表了油微菌科的一个新属和新种，命名为 Tepiditoga spiralis gen. nov.，sp. nov.。
