利用韦氏木层孔菌对玉米秸秆进行微生物预处理，用于酶解和乙醇生产。
Microbial pretreatment of corn stover with Ceriporiopsis subvermispora for enzymatic hydrolysis and ethanol production.
机构信息
Department of Food, Agricultural, and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, Wooster, OH 44691-4096, USA.
出版信息
Bioresour Technol. 2010 Aug;101(16):6398-403. doi: 10.1016/j.biortech.2010.03.070. Epub 2010 Apr 8.
The feasibility of concurrent wet storage and microbial pretreatment of corn stover with Ceriporiopsis subvermispora for ethanol production was investigated in this study. The effects of particle size (5-15 mm), moisture content (45-85%), pretreatment time (18-35 d), and temperature (4-37 degrees C) on lignin degradation and enzymatic hydrolysis yield were studied. The results showed that C. subvermispora selectively degraded lignin up to 31.59% with a limited cellulose loss of less than 6% during an 18-d pretreatment. When 5mm corn stover was pretreated at 28 degrees C with 75% moisture content, overall glucose yields of 57.67%, 62.21%, and 66.61% were obtained with 18-, 28-, and 35-d microbial pretreated corn stover, respectively. For the above conditions, the highest overall ethanol yield of 57.80% was obtained with 35-d-pretreated corn stover. Enzymatic hydrolysis yield was highly related to the lignin removal during microbial pretreatment.
本研究探讨了利用 Ceriporiopsis subvermispora 对玉米秸秆进行湿存和微生物预处理协同作用以生产乙醇的可行性。考察了粒径（5-15mm）、含水量（45-85%）、预处理时间（18-35d）和温度（4-37°C）对木质素降解和酶解产率的影响。结果表明，在 18d 的预处理过程中，C. subvermispora 选择性地降解了高达 31.59%的木质素，而纤维素的损失量不到 6%。当 5mm 玉米秸秆在 28°C、75%含水量条件下预处理时，分别用 18d、28d 和 35d 微生物预处理的玉米秸秆，总葡萄糖产率为 57.67%、62.21%和 66.61%。对于上述条件，用 35d 预处理的玉米秸秆获得了最高的总乙醇产率 57.80%。酶解产率与微生物预处理过程中的木质素去除高度相关。
Zotero 插件