Department of Health Management, University of Prince Edward Island, Charlottetown, PEI, C1A 4P3, Canada.
Prev Vet Med. 2010 Jun 1;95(1-2):32-40. doi: 10.1016/j.prevetmed.2010.03.007. Epub 2010 Apr 9.
Our objective was to determine the risk factors associated with bacteriological quality of bulk tank milk. Bulk tank milk samples were collected from all Prince Edward Island dairy herds (n=235) from March 2005 to March 2007. Biweekly total bacterial, preliminary incubation, laboratory pasteurization, and coliform counts were conducted using a Petrifilm culture system. Data for on-farm risk factors were collected via a mail-out survey which consisted of 4 main sections: (1) general farm demographics and management, (2) cow cleanliness and hygiene, (3) milking procedures and mastitis control, and (4) equipment maintenance and cleaning. Of 235 producers, 153 completed the mail-out survey giving a response rate of 65%. Both total aerobic and preliminary incubation counts were positively associated with the amount of soiling on the teats prior to udder preparation, manual cleaning of the bulk tank, and the use of a specific type of detergent. Additionally, various methods of premilking udder preparation were important, with pre-dip followed by drying being superior to other methods in reducing the bacterial counts. The laboratory pasteurization count was positively associated with the presence of a plate cooler and inadequate frequency of acid wash, whereas having a water purification system was negatively associated with laboratory pasteurization count. Finally, coliform count was negatively associated with clipping udder hair and automated washing of the bulk tank, whereas increasing herd size and inadequate frequency of acid wash were risk factors. Season was a significant predictor for all bacterial counts with the lowest counts tending to occur in winter.
我们的目的是确定与散装奶细菌质量相关的风险因素。2005 年 3 月至 2007 年 3 月期间,我们从爱德华王子岛的所有奶牛场(n=235)采集了散装奶样本。使用 Petrifilm 培养系统进行了两周一次的总细菌计数、初步孵育、实验室巴氏杀菌和大肠菌群计数。通过邮寄调查收集了农场风险因素的数据,调查分为 4 个主要部分:(1)农场一般人口统计学和管理,(2)奶牛清洁和卫生,(3)挤奶程序和乳腺炎控制,以及(4)设备维护和清洁。在 235 名生产者中,有 153 名完成了邮寄调查,回复率为 65%。总需氧菌和初步孵育计数均与乳房准备前乳头的污染程度、散装奶罐的手动清洁以及特定类型清洁剂的使用呈正相关。此外,各种预挤奶乳房准备方法也很重要,预浸泡后干燥比其他方法更能降低细菌计数。实验室巴氏杀菌计数与板式冷却器的存在和酸清洗频率不足呈正相关,而拥有水净化系统与实验室巴氏杀菌计数呈负相关。最后,大肠菌群计数与剪去乳房毛发和散装奶罐自动清洗呈负相关,而增加畜群规模和酸清洗频率不足是风险因素。季节是所有细菌计数的重要预测因素,冬季的计数最低。