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应用具有持久效果的新产品减少医院环境中的细菌表面污染。

Reduction of bacterial surface contamination in the hospital environment by application of a new product with persistent effect.

机构信息

Department of Clinical Microbiology, Falun Hospital, Falun, Sweden.

出版信息

J Hosp Infect. 2010 Jun;75(2):112-5. doi: 10.1016/j.jhin.2010.02.007. Epub 2010 Apr 10.

Abstract

The benefit of routine surface disinfection in hospitals has been discussed. In this study we have investigated a new product, Appeartex. After application on surfaces a remnant effect is achieved due to the positive charge of the active molecule. We studied the persistent effect of Appeartex one day after application in both an experimental study in the laboratory and a field study in a hospital ward. Surfaces of bedside tables were investigated. In the experimental study, large inocula of >or=10(6)cfu of S. aureus or enterococci were inoculated on to well-defined areas which had been treated/not treated with Appeartex. One hour later, samples were taken with a swab rinse technique. A reduction in the number of viable bacteria in the magnitude 10-10(3) cfu was seen due to the effect of Appeartex. In the field study the effect on naturally occurring low level contamination was studied. Defined surfaces on bedside tables used by patients were treated/not treated with Appeartex. One day later, samples were taken with contact agar plates and with a new swab method using two sequential nylon flocked swabs. Significantly fewer bacteria were found on Appeartex-treated surfaces compared with untreated surfaces. The median counts on Appeartex-treated surfaces were 10 cfu/50 cm(2), and on untreated surfaces 20 cfu/50 cm(2). There was no significant difference in the number of bacteria found by culture of samples taken with the contact agar method compared with samples taken using the nylon flocked swab method.

摘要

医院内常规表面消毒的益处一直存在争议。在本研究中,我们调查了一种新产品 Appeartex。由于活性分子带正电荷,其在表面应用后会产生残留效应。我们研究了 Appeartex 在应用一天后的持久效果,在实验室的一项实验研究和医院病房的现场研究中都进行了研究。我们调查了床头柜的表面。在实验研究中,我们在已用/未用 Appeartex 处理的明确区域接种了 >or=10(6)cfu 的金黄色葡萄球菌或肠球菌的大接种体。1 小时后,用拭子冲洗技术取样。由于 Appeartex 的作用,可见活细菌数量减少了 10-10(3)cfu 的数量级。在现场研究中,我们研究了对自然存在的低水平污染的影响。使用患者使用的床头柜上的定义表面用 Appeartex 处理/未处理。一天后,使用接触琼脂平板和使用两个连续尼龙植绒拭子的新拭子方法进行取样。与未处理表面相比,在 Appeartex 处理的表面上发现的细菌明显减少。Appeartex 处理表面的中位数计数为 10 cfu/50 cm(2),未处理表面的中位数计数为 20 cfu/50 cm(2)。用接触琼脂法取样的细菌数量与用尼龙植绒拭子法取样的细菌数量没有显著差异。

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