捷克共和国金黄色葡萄球菌菌株中优势存在的两种脱落毒素 A 转换噬菌体谱系的基因组多样性。

Genomic diversity of two lineages of exfoliative toxin A-converting phages predominating in Staphylococcus aureus strains in the Czech Republic.

机构信息

Department of Genetics and Molecular Biology, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlárská 2, 611 37 Brno, Czech Republic.

出版信息

Res Microbiol. 2010 May;161(4):260-7. doi: 10.1016/j.resmic.2010.03.008. Epub 2010 Apr 9.

DOI:10.1016/j.resmic.2010.03.008

PMID:20382218

Abstract

We have isolated and characterized two distinct types of exfoliative toxin A (ETA)-converting bacteriophages originating from Staphylococcus aureus strains responsible for massive outbreaks of pemphigus neonatorum in the Czech Republic. Three induced phages designated as ph iB531, phi B557 and phi B122 were found to be capable of transferring the eta gene into the prophageless non-toxigenic S. aureus strain and converting it into an ETA producer. Comparisons of the phage sequences derived from 12 selected genes and 2 genomic segments (polymorphic P2 and conserved C4) revealed that phi B531 and phi B557 were identical each other, but phi B122 differed from them in 5 gene sequences, the xis gene content and the virion protein profile. Thus, phi B122 represents a new type of still undescribed ETA-converting phage. This study highlights not only the conclusive genomic diversity of eta gene-positive phages, but also their virulence implications in impetigo S. aureus strains.

摘要

我们已经从导致捷克共和国新生儿天疱疮大规模爆发的金黄色葡萄球菌菌株中分离和鉴定了两种不同类型的脱落毒素 A (ETA) 转化噬菌体。发现三种诱导噬菌体分别命名为 phiB531、phiB557 和 phiB122，能够将 eta 基因转移到无前噬菌体的非产毒金黄色葡萄球菌菌株中，并将其转化为 ETA 产生菌。对来自 12 个选定基因和 2 个基因组片段（多态性 P2 和保守 C4）的噬菌体序列的比较表明，phiB531 和 phiB557 彼此完全相同，但 phiB122 在 5 个基因序列、xis 基因含量和病毒蛋白图谱方面与它们不同。因此，phiB122 代表了一种尚未描述的新型 ETA 转化噬菌体。本研究不仅强调了 eta 基因阳性噬菌体的结论性基因组多样性，还强调了它们在脓疱疮金黄色葡萄球菌菌株中的毒力影响。