Botka Tibor, Růžičková Vladislava, Svobodová Karla, Pantůček Roman, Petráš Petr, Čejková Darina, Doškař Jiří
Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
Int J Med Microbiol. 2017 Sep;307(6):291-296. doi: 10.1016/j.ijmm.2017.05.005. Epub 2017 May 29.
Exfoliative toxin B (ETB) encoded by some large plasmids plays a crucial role in epidermolytic diseases caused by Staphylococcus aureus. We have found as yet unknown types of etb gene-positive plasmids isolated from a set of impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Plasmids from the strains of clonal complex CC121 were related to archetypal plasmid pETB. Sharing a 33-kb core sequence including virulence genes for ETB, EDIN C, and lantibiotics, they were assigned to a stand-alone lineage, named pETB-based plasmids. Differing from each other in the content of variable DNA regions, they formed four sequence types. In addition to them, a novel unique plasmid pETB608 isolated from a strain of ST130 was described. Carrying conjugative cluster genes, as well as new variants of etb and edinA genes, pETB608 could be regarded as a source of a new lineage of ETB plasmids. We have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.
一些大质粒编码的剥脱毒素B（ETB）在金黄色葡萄球菌引起的表皮松解性疾病中起关键作用。我们从捷克妇产医院新生儿脓疱病暴发中涉及的一组脓疱疮菌株中分离出了迄今未知类型的etb基因阳性质粒。克隆复合体CC121菌株的质粒与原型质粒pETB相关。它们共享一个33 kb的核心序列，包括ETB、EDIN C和羊毛硫抗生素的毒力基因，被归为一个独立的谱系，命名为基于pETB的质粒。它们在可变DNA区域的内容上彼此不同，形成了四种序列类型。除此之外，还描述了从ST130菌株中分离出的一种新型独特质粒pETB608。pETB608携带接合簇基因以及etb和edinA基因的新变体，可被视为ETB质粒新谱系的来源。我们设计了一种有用的检测方法，有助于精确鉴定所有描述的ETB质粒类型。
