Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 61137 Brno, Czech Republic.
Diagn Microbiol Infect Dis. 2010 Mar;66(3):248-52. doi: 10.1016/j.diagmicrobio.2009.10.008. Epub 2009 Nov 10.
The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various phiETA prophages of serogroup B responsible for dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-positive strains into 6 groups designated ETA-B1, ETA-B2, ETA-B3, ETA-B4, ETA-B5, and ETA-B6. The method was tested on a diverse set of 101 ETA and/or ETB-positive S. aureus strains isolated in 22 Czech maternity hospitals and 1 Slovak maternity hospital between 1998 and 2009. This novel PCR strategy is reliable in the rapid identification of yet undescribed ETA-converting B prophages and differentiation of the closely related ETA-positive strains, and it is a convenient tool for hospital epidermolytic infection control.
剥脱性毒素 A (ETA) 由位于金黄色葡萄球菌噬菌体上的基因编码。我们开发了一种单反应多重聚合酶链反应 (PCR) 检测方法，用于快速、特异性检测导致 eta 基因传播和临床菌株 ETA 产生的 B 群各种 phiETA 噬菌体。这种 PCR 策略可将 ETA 阳性菌株分为 6 组，分别命名为 ETA-B1、ETA-B2、ETA-B3、ETA-B4、ETA-B5 和 ETA-B6。该方法在 1998 年至 2009 年间捷克的 22 家妇产医院和 1 家斯洛伐克妇产医院分离的 101 株 ETA 和/或 ETB 阳性金黄色葡萄球菌菌株的多样化组上进行了测试。这种新的 PCR 策略可快速鉴定尚未描述的 ETA 转化 B 噬菌体，并区分密切相关的 ETA 阳性菌株，是医院表皮松解感染控制的便捷工具。
Zotero 插件