基于 phiETA 噬菌体多态性快速检测和区分产表皮剥脱毒素 A 的金黄色葡萄球菌菌株。

Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms.

机构信息

Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 61137 Brno, Czech Republic.

出版信息

Diagn Microbiol Infect Dis. 2010 Mar;66(3):248-52. doi: 10.1016/j.diagmicrobio.2009.10.008. Epub 2009 Nov 10.

DOI:10.1016/j.diagmicrobio.2009.10.008

PMID:19903585

Abstract

The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various phiETA prophages of serogroup B responsible for dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-positive strains into 6 groups designated ETA-B1, ETA-B2, ETA-B3, ETA-B4, ETA-B5, and ETA-B6. The method was tested on a diverse set of 101 ETA and/or ETB-positive S. aureus strains isolated in 22 Czech maternity hospitals and 1 Slovak maternity hospital between 1998 and 2009. This novel PCR strategy is reliable in the rapid identification of yet undescribed ETA-converting B prophages and differentiation of the closely related ETA-positive strains, and it is a convenient tool for hospital epidermolytic infection control.

摘要

剥脱性毒素 A (ETA) 由位于金黄色葡萄球菌噬菌体上的基因编码。我们开发了一种单反应多重聚合酶链反应 (PCR) 检测方法，用于快速、特异性检测导致 eta 基因传播和临床菌株 ETA 产生的 B 群各种 phiETA 噬菌体。这种 PCR 策略可将 ETA 阳性菌株分为 6 组，分别命名为 ETA-B1、ETA-B2、ETA-B3、ETA-B4、ETA-B5 和 ETA-B6。该方法在 1998 年至 2009 年间捷克的 22 家妇产医院和 1 家斯洛伐克妇产医院分离的 101 株 ETA 和/或 ETB 阳性金黄色葡萄球菌菌株的多样化组上进行了测试。这种新的 PCR 策略可快速鉴定尚未描述的 ETA 转化 B 噬菌体，并区分密切相关的 ETA 阳性菌株，是医院表皮松解感染控制的便捷工具。