Garman Elspeth F
Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England.
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):339-51. doi: 10.1107/S0907444910008656. Epub 2010 Mar 24.
Radiation damage inflicted during diffraction data collection in macromolecular crystallography has re-emerged in the last decade as a major experimental and computational challenge, as even for crystals held at 100 K it can result in severe data-quality degradation and the appearance in solved structures of artefacts which affect biological interpretations. Here, the observable symptoms and basic physical processes involved in radiation damage are described and the concept of absorbed dose as the basic metric against which to monitor the experimentally observed changes is outlined. Investigations into radiation damage in macromolecular crystallography are ongoing and the number of studies is rapidly increasing. The current literature on the subject is compiled as a resource for the interested researcher.
在过去十年中，大分子晶体学衍射数据收集过程中造成的辐射损伤再次成为一个重大的实验和计算挑战，因为即使对于保存在100 K的晶体，它也可能导致严重的数据质量下降，并在解析结构中出现影响生物学解释的伪影。本文描述了辐射损伤中涉及的可观察症状和基本物理过程，并概述了吸收剂量的概念，作为监测实验观察到的变化的基本指标。对大分子晶体学中辐射损伤的研究正在进行，研究数量正在迅速增加。本文汇编了关于该主题的现有文献，作为感兴趣的研究人员的参考资源。
