开发一种经济有效的方法来测定叶类植物中的硝酸盐和亚硝酸盐含量以及土耳其爱琴海地区生长的一些绿叶蔬菜中的硝酸盐和亚硝酸盐含量。

Development of a cost-effective method for nitrate and nitrite determination in leafy plants and nitrate and nitrite contents of some green leafy vegetables grown in the Aegean region of Turkey.

机构信息

Food Engineering Department, Faculty of Engineering, Ege University, Bornova Izmir, Turkey.

出版信息

J Agric Food Chem. 2010 May 12;58(9):5235-40. doi: 10.1021/jf904558c.

PMID:20384339

Abstract

An accurate, fast, easily applicable, and cost-effective method for the determination of nitrate and nitrite was developed. This method was much more reliable than the cadmium column reduction method, which is a tedious and time-consuming procedure and not easily applicable. The principle of the method was reduction of nitrate to nitrite with cadmium acetate solution and zinc powder and then treatment with Griess reagent. Recovery of the method changed from 92.9 to 102.8%, and detection limit was found as 31.4 mg/kg. Coefficient of variation was 3.16% for intraday precision. Nitrate and nitrite contents of 10 types of leafy vegetables native to the Aegean region of Turkey were determined. Wild radish, chicory, fennel, blessed thistle, blue mallow, and chard were analyzed for the first time. Nitrate contents were found between 354.8 mg/kg for iceberg lettuce and 4653 mg/kg for wild radish. Tested vegetables contained <26.33 mg/kg nitrite.

摘要

建立了一种准确、快速、易于应用且经济有效的硝酸盐和亚硝酸盐测定方法。该方法比镉柱还原法可靠得多，后者繁琐且耗时，不易应用。该方法的原理是用乙酸镉溶液和锌粉将硝酸盐还原为亚硝酸盐，然后用格里斯试剂处理。方法的回收率从 92.9%变为 102.8%，检测限为 31.4mg/kg。日内精密度的变异系数为 3.16%。测定了土耳其爱琴海地区的 10 种叶菜硝酸盐和亚硝酸盐的含量。首次对野萝卜、菊苣、茴香、苦蓟、蓝葵和菠菜进行了分析。硝酸盐含量在 354.8mg/kg 的生菜和 4653mg/kg 的野萝卜之间。所测蔬菜中亚硝酸盐含量<26.33mg/kg。