Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Bioresour Technol. 2010 Aug;101(15):5885-91. doi: 10.1016/j.biortech.2010.02.085. Epub 2010 Apr 10.
Xylan and rice straw were used to produce H(2) via a two-stage approach combining feedstock pretreatment/hydrolysis and dark H(2) fermentation. Acinetobacter junii F6-02 was used to produce cellulolytic enzymes (mainly xylanase) to hydrolyze xylan and pretreated rice straw. The hydrolysates were converted to H(2) by Clostridium butyricum CGS5 via dark fermentation. Investigation of kinetics of xylanase on xylan and NaOH-pretreated rice straw shows nu(max) values of 8.6 and 3.6g/L/h, and K(m) values of 10.6 and 26.9 g/L, respectively. A maximum hydrogen production rate of 62.5 and 26.8 ml/h/L was obtained from hydrolysate of xylan and pretreated rice straw, respectively, while the hydrogen yield was 0.70 and 0.76 mol H(2)/mol xylose, respectively. Simultaneous saccharification and BioH(2) fermentation from xylan was also conducted but giving a lower hydrogen production rate (35.3 ml/h/L) than that of the two-stage process.
木聚糖和稻草被用于通过结合原料预处理/水解和暗发酵的两阶段方法来生产 H(2)。不动杆菌属 Junii F6-02 用于生产纤维素酶（主要是木聚糖酶）来水解木聚糖和预处理过的稻草。水解物通过丁酸梭菌 CGS5 通过暗发酵转化为 H(2)。对木聚糖酶在木聚糖和 NaOH 预处理过的稻草上的动力学研究表明，最大酶活（nu(max)）值分别为 8.6 和 3.6g/L/h，Km 值分别为 10.6 和 26.9 g/L。从木聚糖的水解物和预处理过的稻草中分别获得了 62.5 和 26.8 ml/h/L 的最大产氢速率，而氢产率分别为 0.70 和 0.76 mol H(2)/mol 木糖。也进行了木聚糖的同步糖化和 BioH(2)发酵，但产氢速率（35.3 ml/h/L）低于两阶段工艺。
