Kang Beom-Jung, Jeon Jong-Min, Bhatia Shashi Kant, Kim Do-Hyung, Yang Yung-Hun, Jung Sangwon, Yoon Jeong-Jun
Green & Sustainable Materials R&D Department, Korea Institute of Industrial Technology (KITECH), Chunan-si 31056, Republic of Korea.
Department of Biological Engineering, Konkuk University, Seoul 27478, Republic of Korea.
Polymers (Basel). 2023 Jan 29;15(3):681. doi: 10.3390/polym15030681.
Coffee waste is an abundant biomass that can be converted into high value chemical products, and is used in various renewable biological processes. In this study, oil was extracted from spent coffee grounds (SCGs) and used for polyhydroxyalkanoate (PHA) production through . The oil-extracted SCGs (OESCGs) were hydrolyzed and used for biohydrogen production through DSM10702. The oil extraction yield through -hexane was 14.4%, which accounted for 97% of the oil present in the SCGs. OESCG hydrolysate (OESCGH) had a sugar concentration of 32.26 g/L, which was 15.4% higher than that of the SCG hydrolysate (SCGH) (27.96 g/L). Hydrogen production using these substrates was 181.19 mL and 136.58 mL in OESCGH and SCGH media, respectively. The consumed sugar concentration was 6.77 g/L in OESCGH and 5.09 g/L in SCGH media. VFA production with OESCGH (3.58 g/L) increased by 40.9% compared with SCGH (2.54 g/L). In addition, in a fed-batch culture using the extracted oil, cell dry weight was 5.4 g/L, PHA was 1.6 g/L, and PHA contents were 29.5% at 24 h.
咖啡渣是一种丰富的生物质，可转化为高价值化学产品，并用于各种可再生生物过程。在本研究中，从废咖啡渣（SCGs）中提取油脂，并通过……用于聚羟基脂肪酸酯（PHA）的生产。对提取油脂后的SCGs（OESCGs）进行水解，并通过DSM10702用于生物制氢。通过正己烷提取的油脂产率为14.4%，占SCGs中油脂含量的97%。OESCG水解产物（OESCGH）的糖浓度为32.26 g/L，比SCG水解产物（SCGH，27.96 g/L）高15.4%。在OESCGH和SCGH培养基中，使用这些底物的产氢量分别为181.19 mL和136.58 mL。OESCGH培养基中消耗的糖浓度为6.77 g/L，SCGH培养基中为5.09 g/L。与SCGH（2.54 g/L）相比，OESCGH的挥发性脂肪酸产量（3.58 g/L）增加了40.9%。此外，在使用提取油脂的分批补料培养中，24 h时细胞干重为5.4 g/L，PHA为1.6 g/L，PHA含量为29.5%。
