Research Center for Compact Chemical Process, National Institute of Advanced Industrial Science and Technology (AIST), Nigatake 4-2-1, Miyagino-ku, Sendai, 983-8551, Japan.
Chem Commun (Camb). 2010 May 7;46(17):2941-3. doi: 10.1039/b925607e. Epub 2010 Mar 9.
The assembly of a hetero-protein (Renilla reniformis luciferase (Rluc) and a green fluorescence protein (sGFP)) encapsulated in folded-sheet mesoporous material with 7.1 nm pore diameter (FSM7.1), which was used for studying protein-protein interactions in pores of mesoporous silica, has been confirmed by the detection of bioluminescence resonance energy transfers (BRET).
已通过检测生物发光共振能量转移(BRET)证实,具有 7.1nm 孔径的折叠片状介孔材料(FSM7.1)中封装的异源蛋白(海肾荧光素酶(Rluc)和绿色荧光蛋白(sGFP))的组装,可用于研究介孔硅孔中的蛋白质-蛋白质相互作用。