Zhang Yong-hong, Zhao Yan, Ma Li-na, Shi Ling-xian, Jin Yi, He Zhi-min, Zhang Xiao-dan, Chen Xin-yue
Beijing Youan Hospital, Capital Medical University, Beijing 100069, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Oct;23(5):331-3.
To investigate the effect of proliferation and differentiation of CD8 T cells on the progression in patients co-infected with HIV and HCV.
To address this issue, the presences of CD57 and CD28 in the surface of CD8+ T-cell were monitored using flow cytometry in 20 patients co-infected with HIV and HCV and 20 patients infected with HCV alone. The proliferation and differentiations of CD8+ T cell were compared hetween patients co-infected with HIV and HCV and ones with HCV infection alone, to clarify the association hetween the function of CU and the progression of disease.
A high presence (28.84 +/- 4.49)% of CD57 in the surface of CD8+ T-cell in the patients with HIV/HCV co-infections was found, comparing with a low presence (8.24% +/- 5.05%) of CU57 in the patients with HCV infection alone, the difference hetween these two groups is significant (P < 0.001). Moreover, A clear linear regression hetween the percentage of CD57CD8t T and HCV viral load (log) was identified (P = 0.023, R2 = 0.21). In addition, the differentiations of CD8 T cells were compared between patients with HIV/HCV co-infection and mono-HCV infection: the dominant cells in patients with mono-HCV infection were ones in intermediate stage, while, a late differentiation process of CU8 T cells might he associated with HIV/HCV co-infection.
The differences of proliferation and differentiation of CTL. are significant, between HIV/HCV co- infection and mono-HCV infection. Lower proliferation and late stage of differentiations of CD8 T cell might affect the clearance of hepatitis C virus, weaken CU immunological response and induce chronic inflammation, finally will accelerate the progression of HCV infection.
探讨CD8 T细胞增殖和分化对HIV和HCV合并感染患者病情进展的影响。
为解决此问题，采用流式细胞术监测20例HIV和HCV合并感染患者及20例单纯HCV感染患者CD8+ T细胞表面CD57和CD28的表达情况。比较HIV和HCV合并感染患者与单纯HCV感染患者CD8+ T细胞的增殖和分化情况，以阐明CD8 T细胞功能与疾病进展之间的关联。
发现HIV/HCV合并感染患者CD8+ T细胞表面CD57的高表达率为(28.84±4.49)%，而单纯HCV感染患者CD57的低表达率为(8.24%±5.05%)，两组之间差异有统计学意义(P<0.001)。此外，还确定了CD57+CD8+ T细胞百分比与HCV病毒载量(log)之间存在明显的线性回归关系(P = 0.023，R2 = 0.21)。另外，比较了HIV/HCV合并感染患者和单纯HCV感染患者CD8 T细胞的分化情况：单纯HCV感染患者的主要细胞处于中间阶段，而CD8 T细胞的晚期分化过程可能与HIV/HCV合并感染有关。
HIV/HCV合并感染与单纯HCV感染之间，CTL的增殖和分化差异显著。CD8 T细胞较低的增殖和晚期分化可能会影响丙型肝炎病毒的清除，削弱CD8免疫反应并诱发慢性炎症，最终加速HCV感染的进展。
