2006年中国新疆和田地区甲型肝炎病毒流行株基因分型研究
[Genotyping of hepatitis A virus prevalent strains in Xinjiang Hetian of China in 2006].
作者信息
Yierhali Ayiguli, Cao Jing-yuan, Aili Aideer, Wen Qian, Yang Shi-ping, Ku Re-xi, Meng Qing-ling, Li Xin-lan, Bi Sheng-li
机构信息
Center for Disease Control and Prevention of Xinjiang Uyghur Autonomous Region, Urumqi, Xinjiang 830011, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Oct;23(5):358-60.
OBJECTIVE
To analysis the genotypes of wild type hepatitis A virus circulated in Xinjiang Hetian of China in 2006.
METHODS
The Vp1-2A region of HAV genome was amplified and sequenced from serum samples collected in Xinjiang Hetian of China in 2006, and subjected to phylogenetic analysis by Neighbor Joining (NJ) method.
RESULTS
The nucleotide sequence differences in the VP1-2A region among Xinjiang Hetian HAV strains ranged from 0%-3.9%, all belonged to sub-genotype 1A. Genetically similar strains were identified among Xinjiang Hetian 2006 and Xinjiang Yili 2005 of China isolates. Only 0-2 amino acid differences were found among the Xinjiang Hetian HAV isolates in the VP1-2A region.
CONCLUSION
There were different HAV strains existing in the investigated areas, these strains may have different transmission pathways for the spread of the disease. The results indicate the usefulness of molecular epidemiological methods in studying changes in the circulating HAV strains and in tracing transmission routes, and also for effectively control measures to prevent the spread of the disease.
目的
分析2006年在中国新疆和田地区流行的野生型甲型肝炎病毒的基因型。
方法
对2006年在中国新疆和田地区采集的血清样本进行甲型肝炎病毒基因组Vp1-2A区域的扩增和测序，并采用邻接法（NJ）进行系统发育分析。
结果
新疆和田甲型肝炎病毒株VP1-2A区域的核苷酸序列差异为0%-3.9%，均属于1A亚基因型。在中国2006年新疆和田分离株与2005年新疆伊犁分离株中鉴定出基因相似的毒株。新疆和田甲型肝炎病毒分离株在VP1-2A区域仅发现0-2个氨基酸差异。
结论
在调查地区存在不同的甲型肝炎病毒株，这些毒株可能有不同的传播途径导致疾病传播。结果表明分子流行病学方法在研究流行的甲型肝炎病毒株变化、追踪传播途径以及制定有效防控措施以预防疾病传播方面具有实用性。
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