Nikitchenko Iu V, Dziuba V M, Popovych A S, Bondar V V, Sheremet H O
Ukr Biokhim Zh (1999). 2009 Nov-Dec;81(6):104-10.
The state of enzymatic antioxidant system and thyroid status in the rat tissues under early postnatal overnutrition leading to shortening of life-span and calorie-restricted diet extending lifespan have been studied. It was found that the concentration of thyroxine, body weight and content of lipid hydroperoxides in the blood serum and liver post-mitochondrial fraction of rats had been reliably increased. At the same time the concentration of GSH and GSSG, superoxide dismutase, Se-dependent glutathione peroxidase (GP) activities in the liver and GP activity in the blood serum of experimental rats were reduced. The use of calorie-restricted diet for 2 months showed considerable decrease of thyroxine and triiodothyronine concentration, body weight and the content of lipid hydroperoxides in the blood serum of experimental rats compared with control. At the same time GP activity in the liver, Se-dependent GP activity and content of ceruloplasmin in blood of experimental rats were essentially enhanced. The obtained results allow to conclude that from all studied indices the activity of GSH-dependent antioxidant system (especially Se-dependent GP activity) and thyroid status revealed the most sensible changes in response to the used kind of nutrition. Unlike hypocaloric diet overnutrition results in a decrease of Se-dependent GP activity in the liver and blood of rats. That may be the most crucial moment in the maintenance of prooxidant-antioxidant balance of tissues.
研究了产后早期营养过剩导致大鼠寿命缩短以及热量限制饮食延长寿命的情况下，大鼠组织中酶促抗氧化系统的状态和甲状腺状况。结果发现，大鼠血清和肝脏线粒体后组分中甲状腺素浓度、体重和脂质氢过氧化物含量均显著增加。与此同时，实验大鼠肝脏中谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）的浓度、超氧化物歧化酶、硒依赖性谷胱甘肽过氧化物酶（GP）活性以及血清中GP活性均降低。与对照组相比，实验大鼠采用热量限制饮食2个月后，血清中甲状腺素和三碘甲状腺原氨酸浓度、体重以及脂质氢过氧化物含量均显著降低。与此同时，实验大鼠肝脏中的GP活性、硒依赖性GP活性以及血液中铜蓝蛋白含量均显著增强。所得结果表明，在所研究的所有指标中，谷胱甘肽依赖性抗氧化系统的活性（尤其是硒依赖性GP活性）和甲状腺状况对所用营养类型的反应最为敏感。与低热量饮食不同，营养过剩会导致大鼠肝脏和血液中硒依赖性GP活性降低。这可能是维持组织氧化还原平衡的最关键因素。
