Department of Physiological Sciences, Federal University of São Carlos, São Carlos/SP, Brazil.
Connect Tissue Res. 2010 Dec;51(6):459-66. doi: 10.3109/03008201003676330. Epub 2010 Apr 13.
Tendon remodeling relies on extracellular matrix (ECM) restructuring by the matrix metallopeptidases (MMPs). The aim of this study was to investigate MMP-2 activity in different regions of the calcaneal tendon (CT) after resistance training (RT) in ovariectomized rats. Wistar adult female rats were grouped into sedentary (Sed-Intact), ovariectomized sedentary (Sed-Ovx), acute exercise (AcuteEx-Intact), ovariectomized acute exercise (AcuteEx-Ovx), resistance trained (ChronicEx-Intact), and ovariectomized resistance trained (ChronicEx-Ovx) (n = 10 each group). The RT protocol required the animals to climb a 1.1-m vertical ladder with weights attached to their tail. The sessions were performed once every 3 days with 4-9 climbs and 8-12 dynamic movements per scaling. The acute groups performed one session and the chronic groups underwent 12 weeks of RT. There was an increase in total MMP-2 activity in Sed-Ovx, AcuteEx-Intact, and ChronicEx-Intact compared with that in Sed-Intact in the proximal region of CT. AcuteEx-Ovx exhibited higher total MMP-2 than Sed-Ovx and AcuteEx-Intact in the distal region of CT. Chronic-Ovx presented lower total MMP-2 activity than Sed-Ovx and Chronic-Intact in the distal region of tendon. The active MMP-2 was higher for the AcuteEx-Ovx than Sed-Ovx and AcuteEx-Intact in proximal region of tendon. There was higher active MMP-2 in the distal region of tendon in the Acute-Ovx than in the Sed-Ovx and AcuteEx-Intact. Ovariectomy and resistance exercise modulate MMP-2 activity according to specific tendon region, indicating a differentiated tissue remodeling.
肌腱重塑依赖于细胞外基质（ECM）的基质金属蛋白酶（MMPs）重排。本研究旨在探讨去卵巢大鼠阻力训练（RT）后跟腱（CT）不同部位 MMP-2 活性。Wistar 成年雌性大鼠分为安静（Sed-Intact）、去卵巢安静（Sed-Ovx）、急性运动（AcuteEx-Intact）、去卵巢急性运动（AcuteEx-Ovx）、抗阻训练（ChronicEx-Intact）和去卵巢抗阻训练（ChronicEx-Ovx）（每组 n = 10）。RT 方案要求动物用尾巴上的重物爬上 1.1 米高的垂直梯子。急性组进行一次训练，慢性组进行 12 周 RT，每 3 天进行 1 次，每次 4-9 次攀登和 8-12 次动态运动。与 Sed-Intact 相比，Sed-Ovx、AcuteEx-Intact 和 ChronicEx-Intact 的 CT 近端总 MMP-2 活性增加。与 Sed-Ovx 和 AcuteEx-Intact 相比，AcuteEx-Ovx 在 CT 远端具有更高的总 MMP-2。与 Sed-Ovx 和 Chronic-Intact 相比，Chronic-Ovx 在 CT 远端具有更低的总 MMP-2 活性。与 Sed-Ovx 和 AcuteEx-Intact 相比，急性运动（AcuteEx-Ovx）的活性 MMP-2 在近端肌腱中更高。在跟腱的远端，急性运动（AcuteEx-Ovx）的活性 MMP-2 高于 Sed-Ovx 和 AcuteEx-Intact。去卵巢和阻力运动根据特定的肌腱区域调节 MMP-2 活性，表明组织重塑具有分化性。
