Department of Anatomy, Taipei Medical University, Taipei, Taiwan.
Br J Nutr. 2010 Sep;104(5):686-92. doi: 10.1017/S000711451000111X. Epub 2010 Apr 14.
Arginine (Arg) is known to possess numerous useful physiological properties and have immunomodulatory effects. In vitro studies reported that Arg inhibits advanced glycation endproduct (AGE) formation; however, the effects of Arg on the receptor of AGE (RAGE) expression in inflammatory conditions are not clear. The present study investigated the effects of dietary Arg supplementation on inflammatory mediator production and RAGE expression in type 2 diabetic rats. There were one normal control (NC) group and two diabetic groups in the present study. Rats in the NC group were fed with a regular chow diet. One diabetic group (DM) was fed a common semi-purified diet while the other diabetic group received a diet in which part of the casein was replaced by Arg (DM-Arg) for 8 weeks. Diabetes was induced by an intraperitoneal injection of nicotinamide followed by streptozotocin. Rats with blood glucose levels exceeding 1800 mg/l were considered diabetic. Blood samples and the liver and lungs of the animals were collected at the end of the study for further analysis. Results showed that plasma glucose and fructosamine contents were significantly higher in the diabetic groups than those in the NC group. The DM group had higher fructosamine and C-reactive protein than the DM-Arg group. Immunohistochemical staining showed that the expressions of RAGE in liver and lung tissues were significantly lower in the DM-Arg group than in the DM group. These results suggest that supplemental dietary Arg can decrease AGE-RAGE interactions and consequently reduce tissue damage in rats with type 2 diabetes.
精氨酸（Arg）具有许多有用的生理特性和免疫调节作用。体外研究表明Arg 可抑制晚期糖基化终产物（AGE）的形成；然而，Arg 对炎症条件下 AGE 受体（RAGE）表达的影响尚不清楚。本研究探讨了饮食补充 Arg 对 2 型糖尿病大鼠炎症介质产生和 RAGE 表达的影响。本研究有一个正常对照组（NC）和两个糖尿病组。NC 组大鼠喂食常规饲料。一组糖尿病组（DM）喂食普通半纯化饮食，另一组糖尿病组（DM-Arg）用部分酪蛋白替代 Arg 喂养 8 周。糖尿病通过腹腔注射烟酰胺后注射链脲佐菌素诱导。血糖水平超过 1800mg/l 的大鼠被认为患有糖尿病。研究结束时采集血液样本和动物的肝脏和肺部进行进一步分析。结果表明，糖尿病组的血浆葡萄糖和果糖胺含量明显高于 NC 组。DM 组的果糖胺和 C 反应蛋白均高于 DM-Arg 组。免疫组织化学染色显示，DM-Arg 组肝、肺组织中 RAGE 的表达明显低于 DM 组。这些结果表明，补充饮食 Arg 可以减少 AGE-RAGE 相互作用，从而减少 2 型糖尿病大鼠的组织损伤。
