Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China.
Anal Biochem. 2010 Aug;403(1-2):36-42. doi: 10.1016/j.ab.2010.04.011. Epub 2010 Apr 11.
Bienzyme bionanomultilayer electrode for glucose biosensing was constructed based on functional carbon nanotubes and sugar-lectin biospecific interaction through layer-by-layer (LBL) assembly. After being functionalized by wrapping with polyelectrolyte, multiwalled carbon nanotubes (MCNTs) were water soluble and positively charged. MCNT-bienzyme bionanomultilayer electrode was then fabricated by LBL assembly of horseradish peroxidase (HRP) and glucose oxidase (GOD) on functional MCNT modified electrode. The attachment of the MCNT-bienzyme bionanomultilayer with the underlying electrode and each layer in the bionanomultilayer was based on reliably electrostatic or sugar-lectin biospecific interaction. The developed bienzyme biosensor exhibited fast amperometric response for the determination of glucose. The linear response of the developed biosensor for the determination of glucose ranged from 2.0 x 10(-6) to 1.7 x 10(-4) M with a detection limit of 2.5 x 10(-7) M. The biosensor can be used directly to determine glucose in serum. The construction of the bienzyme biosensor showed potential for the preparation of MCNT-enzyme nanocomposite with controllability and high performance.
基于功能化碳纳米管和糖-凝集素生物特异性相互作用，通过层层（LBL）组装构建了用于葡萄糖生物传感的双酶生物纳米多层电极。经过聚电解质包裹功能化后，多壁碳纳米管（MWCNTs）具有水溶性和正电性。然后，通过在功能化 MWCNT 修饰电极上层层组装辣根过氧化物酶（HRP）和葡萄糖氧化酶（GOD），制备了 MWCNT-双酶生物纳米多层电极。MWCNT-双酶生物纳米多层与底层电极和纳米多层中的每一层的附着基于可靠的静电或糖-凝集素生物特异性相互作用。开发的双酶生物传感器对葡萄糖的测定表现出快速的电流响应。开发的生物传感器用于测定葡萄糖的线性响应范围为 2.0 x 10(-6)至 1.7 x 10(-4) M，检测限为 2.5 x 10(-7) M。该生物传感器可直接用于测定血清中的葡萄糖。双酶生物传感器的构建为制备具有可控性和高性能的 MWCNT-酶纳米复合材料展示了潜力。
