Zhu Bi-Wen, Cai Liang, He Xiao-Peng, Chen Guo-Rong, Long Yi-Tao
Key Laboratory for Advanced Materials & Institute of Fine Chemicals, East China University of Science and Technology, 130 Meilong Rd, Shanghai, 200237 PR China.
Chem Cent J. 2014 Nov 25;8(1):67. doi: 10.1186/s13065-014-0067-y. eCollection 2014.
Construction of electrochemical impedance sensors by the self-assembly technique has become a promising strategy for the 'label-free' detection of protein-ligand interactions. However, previous impedance sensors are devoid of an inherent electrochemical signal, which limits the standardization of the sensors for protein recognition in a reproducible manner.
We designed and synthesized an anthraquinonyl glycoside (AG) where the anthraquinone (AQ) moiety can bind to the surface of a graphene-based working electrode while the glycoside serving as a ligand for lectin. By measuring the inherent voltammetric signal of AQ, the glycosides decorated on the working electrode could be simply quantified to obtain electrodes with a unified signal window. Subsequently, impedance analysis showed that the 'standardized' electrodes gave a reproducible electrochemical response to a selective lectin with no signal variation in the presence of unselective proteins.
Anthraquinone-modified ligands could be used to facilitate the standardization of electrochemical impedance sensors for the reproducible, selective analysis of ligand-protein interactions.
通过自组装技术构建电化学阻抗传感器已成为“无标记”检测蛋白质 - 配体相互作用的一种有前景的策略。然而，先前的阻抗传感器缺乏固有的电化学信号，这限制了传感器以可重复的方式用于蛋白质识别的标准化。
我们设计并合成了一种蒽醌基糖苷（AG），其中蒽醌（AQ）部分可与基于石墨烯的工作电极表面结合，而糖苷作为凝集素的配体。通过测量AQ的固有伏安信号，可以简单地对修饰在工作电极上的糖苷进行定量，以获得具有统一信号窗口的电极。随后，阻抗分析表明，“标准化”电极对选择性凝集素给出了可重复的电化学响应，在存在非选择性蛋白质时无信号变化。
蒽醌修饰的配体可用于促进电化学阻抗传感器的标准化，以对配体 - 蛋白质相互作用进行可重复的选择性分析。
