Bergh K, Olsen P O, Halgunset J, Iversen O J
Department of Microbiology, Regional Hospital, Trondheim, Norway.
Acta Anaesthesiol Scand. 1991 Apr;35(3):267-74. doi: 10.1111/j.1399-6576.1991.tb03286.x.
Septicaemia was induced in anaesthetized and artificially ventilated rabbits. Three groups receiving different doses of Escherichia coli 055:B5 and a control group were studied. The animals were followed for 240 min after the start of the bacterial infusion. One animal in the high-dose and one in the medium-dose group died. Septic shock developed in the high-dose group. The infusion of E. coli led to a severe, sustained neutropenia and a dose-dependent thrombocytopenia. A severe arterial hypoxaemia developed during the infusion of bacteria in three of four animals, and in one of three animals in the high- and medium-dose groups, respectively. Both the levels of C5a in plasma and the accumulation of granulocytes in the lung tissue, as assessed by a morphometric method, were dependent on the dose of bacteria. A strong positive correlation was found between levels of C5a and endotoxin. The levels of both C5a and endotoxin correlated positively to the degree of accumulation of granulocytes in the lung tissue. The results from the present study suggest that the magnitude of bacteraemia and the degree of complement activation are probably important factors determining the course and the severity of septicaemia.
在麻醉并进行人工通气的家兔中诱发败血症。研究了接受不同剂量大肠杆菌055:B5的三组动物和一个对照组。在开始输注细菌后对动物进行240分钟的观察。高剂量组和中剂量组各有一只动物死亡。高剂量组发生了感染性休克。输注大肠杆菌导致严重的、持续性中性粒细胞减少和剂量依赖性血小板减少。在四只动物中的三只以及高剂量组和中剂量组的三只动物中的一只在输注细菌期间出现了严重的动脉低氧血症。通过形态计量学方法评估，血浆中C5a水平和肺组织中粒细胞的积聚均取决于细菌剂量。发现C5a水平与内毒素之间存在强正相关。C5a和内毒素水平均与肺组织中粒细胞的积聚程度呈正相关。本研究结果表明，菌血症的程度和补体激活的程度可能是决定败血症病程和严重程度的重要因素。
